Single osteoblast chemical sensor via non-invasive bio-electronic interface

Mo Yang, Xuan Zhang, Bonnie Kohr, Andre Morgan, Cengiz S. Ozkan

Research output: Journal article publicationConference articleAcademic researchpeer-review

Abstract

The broad-spectrum sensitivity of cell based biosensors offers the capability for detecting known and unknown chemical/biological agents. One cellular parameter that is often measured is the extracellular potential of electrically active cells. Membrane excitability in osteoblasts plays a key role in modulating the electrical activity in the presence of chemical agents. However, the complexity of this signal makes interpretation of the cellular response to a chemical agent difficult to interpret. By analyzing shifts in the signal's power spectrum, it is possible to determine a frequency spectrum also known as Signature Pattern Vectors (SPV) specific to a chemical. We used a 5×5 multiple microelectrode array system to spatially position osteoblast cells, by using a gradient AC field. Fast Fourier Transformation (FFT) analyses were used to extract information pertaining to the frequency of firing from the extracellular potential.
Original languageEnglish
Pages (from-to)137-146
Number of pages10
JournalMaterials Research Society Symposium - Proceedings
Volume782
Publication statusPublished - 1 Dec 2003
Externally publishedYes
EventMicro- and Nanosystems - Boston, MA, United States
Duration: 1 Dec 20033 Dec 2003

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials

Cite this