Extracellular potential is an important parameter which indicates the electrical activity of live cells. Membrane excitability in osteoblasts plays a key role in modulating the electrical activity in the presence of chemical agents. The complexity of cell signal makes interpretation of the cellular response to a chemical agent very difficult. By analyzing shifts in the signal power spectrum, it is possible to determine a frequency spectrum also known as Signature Pattern Vectors (SPY) specific to a chemical. It is also essential to characterize single cell sensitivity and response time for specific chemical agents for developing detect-to-warn biosensors. We used a 4×4 multiple Pt microelectrode array to spatially position single osteoblast cells, by using a gradient AC field. Fast Fourier Transformation (FFT) and Wavelet Transformation (WT) analyses were used to extract information pertaining to the frequency of firing from the extracellular potential.
|Number of pages||6|
|Journal||Materials Research Society Symposium - Proceedings|
|Publication status||Published - 1 Dec 2003|
|Event||Biomicroelectromechanical Systems (BioMEMS) - San Francisco, CA, United States|
Duration: 22 Apr 2003 → 25 Apr 2003
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials