Simultaneous removal of antibiotic resistant bacteria and antibiotic resistance genes by molybdenum carbide assisted electrochemical disinfection

Hao Fang, Yinghan Liu, Pengxiang Qiu, Hai Liang Song, Tao Liu, Jianhua Guo, Shuai Zhang

Research output: Journal article publicationJournal articleAcademic researchpeer-review

17 Citations (Scopus)

Abstract

Considering conventional disinfection methods are not effective in simultaneously removing ARB and ARGs, a novel electrochemical disinfection (ED) process assisted by molybdenum carbide (Mo2C) electrodes was developed in this study. The established ED process was proved to effectively inactivate multi-resistant ARB (i.e. Escherichia coli K-12 LE392 with resistance to kanamycin, ampicillin, and tetracycline) and to degrade ARGs (including tetA and blaTEM in the form of both intracellular (iARGs) and extracellular ARGs (eARGs)). Specifically, within 15 min treatment by the Mo2C-assisted ED under 2.0 V, a 5-log ARB removal was realized, without any ARB regrowth observed, indicating a permanent inactivation of ARB by the process. Moreover, degradation of the iARGs (0.4-log reduction of the blaTEM and 3.1-log reduction of the tetA) and the eARGs (4.2-log reduction of the blaTEM and 1.1-log reduction of the tetA) were achieved within 60 min, further underpinning the viability of the Mo2C-based ED. While e-, H2O2, and •O2 played leading roles in the entire process of ED, H+ and •OH contributed to bacterial inactivation in the early and late stages of ED, respectively. The reactive species induced by electrolysis posed pressure to the ARB strains, which enhanced oxidative stress response, triggered higher reactive oxygen species generation, induced membrane damage and changed cellular structure. Collectively, the Mo2C-assisted ED demonstrated in the present study represents an attractive alternative to the traditional disinfection methods in combating the spread of antibiotic resistance.

Original languageEnglish
Article number128733
JournalJournal of Hazardous Materials
Volume432
DOIs
Publication statusPublished - 15 Jun 2022
Externally publishedYes

Keywords

  • Antibiotic resistance genes
  • Antibiotic resistant bacteria
  • Electrochemical disinfection
  • Molybdenum carbide
  • Reactive oxygen species

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution
  • Health, Toxicology and Mutagenesis

Fingerprint

Dive into the research topics of 'Simultaneous removal of antibiotic resistant bacteria and antibiotic resistance genes by molybdenum carbide assisted electrochemical disinfection'. Together they form a unique fingerprint.

Cite this