Simultaneous assessment of autophagy and apoptosis using multispectral imaging cytometry

Claire De La Calle, Pierre Emmanuel Joubert, Ka Wai Helen Law, Milena Hasan, Matthew L. Albert

Research output: Journal article publicationJournal articleAcademic researchpeer-review

28 Citations (Scopus)

Abstract

Multiple stress pathways result in the induction of autophagy and apoptosis. Current methods (e.g., protein gel blot, microscopy) do not offer quantitative single-cell resolution, thus making it difficult to discern if these pathways are mutually exclusive or, in some situations, cooperative in executing cell death. We report a novel method that enables high-throughput, high-content assessment of LC3 puncta and caspase-3 cleavage at the single cell level.
Original languageEnglish
Pages (from-to)1045-1051
Number of pages7
JournalAutophagy
Volume7
Issue number9
DOIs
Publication statusPublished - 1 Jan 2011
Externally publishedYes

Keywords

  • Apoptosis
  • Autophagy
  • Influenza
  • Multispectral imaging cytometry
  • New methodology

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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