Simultaneous assessment of autophagy and apoptosis using multispectral imaging cytometry

Claire De La Calle; Pierre Emmanuel Joubert; Ka Wai Helen Law; Milena Hasan; Matthew L. Albert

doi:10.4161/auto.7.9.16252

Simultaneous assessment of autophagy and apoptosis using multispectral imaging cytometry

Claire De La Calle, Pierre Emmanuel Joubert, Ka Wai Helen Law, Milena Hasan, Matthew L. Albert

Research output: Journal article publication › Journal article › Academic research › peer-review

31 Citations (Scopus)

Abstract

Multiple stress pathways result in the induction of autophagy and apoptosis. Current methods (e.g., protein gel blot, microscopy) do not offer quantitative single-cell resolution, thus making it difficult to discern if these pathways are mutually exclusive or, in some situations, cooperative in executing cell death. We report a novel method that enables high-throughput, high-content assessment of LC3 puncta and caspase-3 cleavage at the single cell level.

Original language	English
Pages (from-to)	1045-1051
Number of pages	7
Journal	Autophagy
Volume	7
Issue number	9
DOIs	https://doi.org/10.4161/auto.7.9.16252
Publication status	Published - 1 Jan 2011
Externally published	Yes

Keywords

Apoptosis
Autophagy
Influenza
Multispectral imaging cytometry
New methodology

ASJC Scopus subject areas

Molecular Biology
Cell Biology

More information

10.4161/auto.7.9.16252

Cite this

@article{5010a25c541641949b548792934f8a6d,

title = "Simultaneous assessment of autophagy and apoptosis using multispectral imaging cytometry",

abstract = "Multiple stress pathways result in the induction of autophagy and apoptosis. Current methods (e.g., protein gel blot, microscopy) do not offer quantitative single-cell resolution, thus making it difficult to discern if these pathways are mutually exclusive or, in some situations, cooperative in executing cell death. We report a novel method that enables high-throughput, high-content assessment of LC3 puncta and caspase-3 cleavage at the single cell level.",

keywords = "Apoptosis, Autophagy, Influenza, Multispectral imaging cytometry, New methodology",

author = "{De La Calle}, Claire and Joubert, {Pierre Emmanuel} and Law, {Ka Wai Helen} and Milena Hasan and Albert, {Matthew L.}",

year = "2011",

month = jan,

day = "1",

doi = "10.4161/auto.7.9.16252",

language = "English",

volume = "7",

pages = "1045--1051",

journal = "Autophagy",

issn = "1554-8627",

publisher = "Landes Bioscience",

number = "9",

}

TY - JOUR

T1 - Simultaneous assessment of autophagy and apoptosis using multispectral imaging cytometry

AU - De La Calle, Claire

AU - Joubert, Pierre Emmanuel

AU - Law, Ka Wai Helen

AU - Hasan, Milena

AU - Albert, Matthew L.

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Multiple stress pathways result in the induction of autophagy and apoptosis. Current methods (e.g., protein gel blot, microscopy) do not offer quantitative single-cell resolution, thus making it difficult to discern if these pathways are mutually exclusive or, in some situations, cooperative in executing cell death. We report a novel method that enables high-throughput, high-content assessment of LC3 puncta and caspase-3 cleavage at the single cell level.

AB - Multiple stress pathways result in the induction of autophagy and apoptosis. Current methods (e.g., protein gel blot, microscopy) do not offer quantitative single-cell resolution, thus making it difficult to discern if these pathways are mutually exclusive or, in some situations, cooperative in executing cell death. We report a novel method that enables high-throughput, high-content assessment of LC3 puncta and caspase-3 cleavage at the single cell level.

KW - Apoptosis

KW - Autophagy

KW - Influenza

KW - Multispectral imaging cytometry

KW - New methodology

UR - http://www.scopus.com/inward/record.url?scp=80052382157&partnerID=8YFLogxK

U2 - 10.4161/auto.7.9.16252

DO - 10.4161/auto.7.9.16252

M3 - Journal article

VL - 7

SP - 1045

EP - 1051

JO - Autophagy

JF - Autophagy

SN - 1554-8627

IS - 9

ER -

Simultaneous assessment of autophagy and apoptosis using multispectral imaging cytometry

Abstract

Keywords

ASJC Scopus subject areas

More information

Other files and links

Cite this