Abstract
Multiple stress pathways result in the induction of autophagy and apoptosis. Current methods (e.g., protein gel blot, microscopy) do not offer quantitative single-cell resolution, thus making it difficult to discern if these pathways are mutually exclusive or, in some situations, cooperative in executing cell death. We report a novel method that enables high-throughput, high-content assessment of LC3 puncta and caspase-3 cleavage at the single cell level.
Original language | English |
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Pages (from-to) | 1045-1051 |
Number of pages | 7 |
Journal | Autophagy |
Volume | 7 |
Issue number | 9 |
DOIs | |
Publication status | Published - 1 Jan 2011 |
Externally published | Yes |
Keywords
- Apoptosis
- Autophagy
- Influenza
- Multispectral imaging cytometry
- New methodology
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology