Abstract
A facile silica coating significantly enhances the thermal stability and polymerase chain reaction (PCR) compatibility of oligonucleotide-gold nanoparticle conjugates, thus enabling colorimetric detection of PCR results in a closed-tube format. This method is specific, sensitive, and generally applicable. Its simplicity, visual readout, and carryover contamination-free features hold promise for point-of-care or on-site DNA testing. KGaA, Weinheim.
| Original language | English |
|---|---|
| Pages (from-to) | 214-219 |
| Number of pages | 6 |
| Journal | Small |
| Volume | 8 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 23 Jan 2012 |
Keywords
- colorimetric detection
- DNA recognition
- gold nanoparticles
- polymerase chain reaction
- silica coating
ASJC Scopus subject areas
- Biotechnology
- Biomaterials
- Engineering (miscellaneous)