Silica-modified oligonucleotide-gold nanoparticle conjugate enables closed-tube colorimetric polymerase chain reaction

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20 Citations (Scopus)

Abstract

A facile silica coating significantly enhances the thermal stability and polymerase chain reaction (PCR) compatibility of oligonucleotide-gold nanoparticle conjugates, thus enabling colorimetric detection of PCR results in a closed-tube format. This method is specific, sensitive, and generally applicable. Its simplicity, visual readout, and carryover contamination-free features hold promise for point-of-care or on-site DNA testing. KGaA, Weinheim.
Original languageEnglish
Pages (from-to)214-219
Number of pages6
JournalSmall
Volume8
Issue number2
DOIs
Publication statusPublished - 23 Jan 2012

Keywords

  • colorimetric detection
  • DNA recognition
  • gold nanoparticles
  • polymerase chain reaction
  • silica coating

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Engineering (miscellaneous)

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