Sichuan pepper attenuates H2O2-induced apoptosis via antioxidant activity and up-regulating heme oxygenase-1 gene expression in primary rat hepatocytes

Huan Zhang, Xuelin Zhou, Marcus Ho Yin Wong, Ka Yi Man, Wing Kwan Pin, John Hok Keung Yeung, Yiu Wa Kwan, George Pak Heng Leung, Pui Man Hoi, Simon Ming Yuen Lee, Chi On Chan, Kam Wah Mok, Peter Hoi Fu Yu, Shun Wan Chan

Research output: Journal article publicationJournal articleAcademic researchpeer-review

2 Citations (Scopus)

Abstract

Sichuan pepper, a well-known traditional Chinese spice, is popularly used in Chinese cuisine. In this study, the antioxidant activities of Sichuan pepper water extract (SP) were evaluated both chemically [in 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH) assay] and biologically [in 2,2′-azobis-(2-amidinopropane) (AAPH)-induced haemolysis assay]. The protective effect of SP against H2O2-induced cytotoxicity in primary Sprague Dawley rat hepatocytes was investigated by using cell viability assay, nuclear staining, intracellular reactive oxygen species (ROS) measurement and flow cytometry. It was found to possess high total phenolic content as assessed by the Folin–Ciocalteu method. The antiapoptotic effect of SP may be mediated, in part, by significantly inhibiting intracellular ROS generation and depletion of the mitochondrial membrane potential. SP was also found to enhance the cellular antioxidant defense system through up-regulating heme oxygenase-1 expression. SP is an effective antioxidant that can be consumed regularly as a functional food for the prevention of oxidative stress-related liver diseases. Practical applications: The present study demonstrated that Sichuan pepper water extract (SP) could enhance the cellular antioxidant ability in hepatocytes. SP is an effective antioxidant that can be consumed regularly as a functional food for the prevention of oxidative stress-related liver diseases.
Original languageEnglish
Article numbere12403
JournalJournal of Food Biochemistry
Volume41
Issue number6
DOIs
Publication statusPublished - 1 Dec 2017

Keywords

  • liver
  • reactive oxygen species
  • Rutaceae
  • Zanthoxylum bungeanum Maxim

ASJC Scopus subject areas

  • Biophysics
  • Food Science
  • Pharmacology
  • Cell Biology

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