Short- to mid-term effects of ovariectomy on bone turnover, bone mass and bone strength in rats

Yan Zhang, Wan Ping Lai, Ping Chung Leung, Chun Fu Wu, Man Sau Wong

Research output: Journal article publicationJournal articleAcademic researchpeer-review

47 Citations (Scopus)

Abstract

To determine the short- to mid-term effects of ovariectomy on bone turnover, bone mass and bone strength in rats. SD rats aged 12 weeks were randomly divided into No-treatment, Sham and OVX groups. The rats were sacrificed for sample collection at week 0, week 4 and week 18 after surgical operation. Chemistries in serum and urine were measured by standard colorimetric methods and bone turnover markers were measured by ELISA kits. Bone mass and bone strength were determined using pQCT system and three-point bending tests, respectively. At week 4, OVX rats showed drastic increase in the level of urine Ca, P and DPD. At week 18, in OVX rats the levels of serum ALP, urine DPD and Ca were much higher and the level of serum Ca was much lower when comparing with Sham rats. Ovariectomy produced significant reduction in cancellous BMD, total BMD and SSI of proximal tibial metaphysis rapidly at week 4 and continuously at week 18 after surgical operation. However, no marked changes of bone mass and bone strength were found in the diaphysis of tibia and femur, respectively. The current study concluded that ovariectomy induced the uncoupling of bone turnover, and the proximal metaphysis of long bone was the sensitive site for the short- to mid-term effect of ovariectomy, demonstrated as the markers of bone mass and stress strain index.
Original languageEnglish
Pages (from-to)898-903
Number of pages6
JournalBiological and Pharmaceutical Bulletin
Volume30
Issue number5
DOIs
Publication statusPublished - 1 May 2007

Keywords

  • Bone mass
  • Bone strength
  • Bone turnover
  • Osteoporosis
  • Ovariectomy

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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