SENSITIVE DIAGNOSIS OF BACTERIAL INFECTIONS WITH LABEL-FREE MICROFLUIDICS

Junchen Liao; Song Lin Chua; Bee Luan Khoo

SENSITIVE DIAGNOSIS OF BACTERIAL INFECTIONS WITH LABEL-FREE MICROFLUIDICS

Junchen Liao
, Song Lin Chua
, Bee Luan Khoo

The Hong Kong Polytechnic University

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

Abstract

Pathogens may hide inside immune cells to avoid the detection of human infectious diseases. We revealed that intracellular bacteria cause monocytes to become much stiffer and larger than healthy individuals. Hence, we introduced a label-free microchip that detects and quantifies monocytes with internalized pathogens (MIP) using inertial focusing. Different bacteria and clinical isolates were utilized to validate the device's functionality and set the infected sample threshold. The MIP platform can complete processing within 1.5 h. Our proof-of-concept shows that the MIP device has excellent prospects for diagnosing microbial infection.

Original language	English
Title of host publication	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	785-786
Number of pages	2
ISBN (Electronic)	9781733419031
Publication status	Published - Oct 2021
Event	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States Duration: 10 Oct 2021 → 14 Oct 2021

Publication series

Name	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/Territory	United States
City	Palm Springs, Virtual
Period	10/10/21 → 14/10/21

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Diagnosis
Intracellular pathogens
Label-free
Microfluidics

ASJC Scopus subject areas

Bioengineering
Chemical Engineering (miscellaneous)

Cite this

Liao, J., Chua, S. L., & Khoo, B. L. (2021). SENSITIVE DIAGNOSIS OF BACTERIAL INFECTIONS WITH LABEL-FREE MICROFLUIDICS. In MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 785-786). (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.

Liao, Junchen ; Chua, Song Lin ; Khoo, Bee Luan. / SENSITIVE DIAGNOSIS OF BACTERIAL INFECTIONS WITH LABEL-FREE MICROFLUIDICS. MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. pp. 785-786 (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

@inproceedings{4f7b36c72cbb4f8c91f6c81b1c3b9b7b,

title = "SENSITIVE DIAGNOSIS OF BACTERIAL INFECTIONS WITH LABEL-FREE MICROFLUIDICS",

abstract = "Pathogens may hide inside immune cells to avoid the detection of human infectious diseases. We revealed that intracellular bacteria cause monocytes to become much stiffer and larger than healthy individuals. Hence, we introduced a label-free microchip that detects and quantifies monocytes with internalized pathogens (MIP) using inertial focusing. Different bacteria and clinical isolates were utilized to validate the device's functionality and set the infected sample threshold. The MIP platform can complete processing within 1.5 h. Our proof-of-concept shows that the MIP device has excellent prospects for diagnosing microbial infection.",

keywords = "Diagnosis, Intracellular pathogens, Label-free, Microfluidics",

author = "Junchen Liao and Chua, \{Song Lin\} and Khoo, \{Bee Luan\}",

note = "Funding Information: This study was supported by the Research Grants Council (RGC). This work was also supported by the City University of Hong Kong and the Hong Kong Polytechnic University, Environmental and Conservation Fund, and State Key Laboratory for Chemical Biology and Drug Discovery. Publisher Copyright: {\textcopyright} 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.; 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 ; Conference date: 10-10-2021 Through 14-10-2021",

year = "2021",

month = oct,

language = "English",

series = "MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

pages = "785--786",

booktitle = "MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

}

Liao, J, Chua, SL & Khoo, BL 2021, SENSITIVE DIAGNOSIS OF BACTERIAL INFECTIONS WITH LABEL-FREE MICROFLUIDICS. in MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 785-786, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021, Palm Springs, Virtual, United States, 10/10/21.

SENSITIVE DIAGNOSIS OF BACTERIAL INFECTIONS WITH LABEL-FREE MICROFLUIDICS. / Liao, Junchen; Chua, Song Lin; Khoo, Bee Luan.
MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. p. 785-786 (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

TY - GEN

T1 - SENSITIVE DIAGNOSIS OF BACTERIAL INFECTIONS WITH LABEL-FREE MICROFLUIDICS

AU - Liao, Junchen

AU - Chua, Song Lin

AU - Khoo, Bee Luan

N1 - Funding Information: This study was supported by the Research Grants Council (RGC). This work was also supported by the City University of Hong Kong and the Hong Kong Polytechnic University, Environmental and Conservation Fund, and State Key Laboratory for Chemical Biology and Drug Discovery. Publisher Copyright: © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

PY - 2021/10

Y1 - 2021/10

N2 - Pathogens may hide inside immune cells to avoid the detection of human infectious diseases. We revealed that intracellular bacteria cause monocytes to become much stiffer and larger than healthy individuals. Hence, we introduced a label-free microchip that detects and quantifies monocytes with internalized pathogens (MIP) using inertial focusing. Different bacteria and clinical isolates were utilized to validate the device's functionality and set the infected sample threshold. The MIP platform can complete processing within 1.5 h. Our proof-of-concept shows that the MIP device has excellent prospects for diagnosing microbial infection.

AB - Pathogens may hide inside immune cells to avoid the detection of human infectious diseases. We revealed that intracellular bacteria cause monocytes to become much stiffer and larger than healthy individuals. Hence, we introduced a label-free microchip that detects and quantifies monocytes with internalized pathogens (MIP) using inertial focusing. Different bacteria and clinical isolates were utilized to validate the device's functionality and set the infected sample threshold. The MIP platform can complete processing within 1.5 h. Our proof-of-concept shows that the MIP device has excellent prospects for diagnosing microbial infection.

KW - Diagnosis

KW - Intracellular pathogens

KW - Label-free

KW - Microfluidics

UR - https://www.scopus.com/pages/publications/85136921531

M3 - Conference article published in proceeding or book

AN - SCOPUS:85136921531

T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

SP - 785

EP - 786

BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021

Y2 - 10 October 2021 through 14 October 2021

ER -

Liao J, Chua SL, Khoo BL. SENSITIVE DIAGNOSIS OF BACTERIAL INFECTIONS WITH LABEL-FREE MICROFLUIDICS. In MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2021. p. 785-786. (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

SENSITIVE DIAGNOSIS OF BACTERIAL INFECTIONS WITH LABEL-FREE MICROFLUIDICS

Abstract

Publication series

Conference

UN SDGs

Keywords

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this