Sensitive and inexpensive molecular test for falciparum malaria: Defecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification

Leo L.M. Poon, Bonnie W.Y. Wong, Edmund H.T. Ma, Kwok H. Chan, Ming Cheung Chow, Wimal Abeyewickreme, Noppadon Tangpukdee, Kwok Y. Yuen, Yi Guan, Sornchai Looareesuwan, J. S.Malik Peiris

Research output: Journal article publicationJournal articleAcademic researchpeer-review

363 Citations (Scopus)

Abstract

Background: Malaria is one of the most important parasitic infections in humans. A sensitive diagnostic test for malaria that could be applied at the community level could be useful in programs to control the disease. The aim of the present work was to develop a simple, inexpensive molecular test for Plasmodium falciparum. Methods: Blood was collected from controls (n = 100) and from patients diagnosed with falciparum malaria infection (n = 102), who were recruited to the study. Heat-treated blood samples were tested by a loop-mediated isothermal amplification (LAMP) assay for P. falciparum. Results were interpreted by a turbidity meter in real time or visually at the end of the assay. To evaluate the assay, DNA from these samples was purified and tested by PCR. Results from the LAMP and PCR assays were compared. Results: The LAMP assay detected P. falciparum directly from heat-treated blood. The quantitative data from the assay correlated to the parasite counts obtained by blood-film microscopic analyses. When we used the PCR assay as the comparison method, the sensitivity and specificity of the LAMP assay were 95% and 99%, respectively. Conclusions: Unlike PCR, the LAMP assay does not require purified DNA for efficient DNA amplification, thereby reducing the cost and turnaround time for P. falciparum diagnosis. The assay requires only basic instruments, and assay positivity can be verified by visual inspection.
Original languageEnglish
Pages (from-to)303-306
Number of pages4
JournalClinical Chemistry
Volume52
Issue number2
DOIs
Publication statusPublished - 1 Feb 2006

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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