TY - JOUR
T1 - Self-protonating, plasma polymerized, superimposed multi-layered biomolecule nanoreservoir as blood-contacting surfaces
AU - Wang, Wenxuan
AU - Lu, Lei
AU - Bei, Ho Pan
AU - Li, Xiangyang
AU - Du, Zeyu
AU - Maitz, Manfred F.
AU - Huang, Nan
AU - Tu, Qiufen
AU - Zhao, Xin
AU - Yang, Zhilu
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (Project 82072072 ), International Cooperation Project by Science and Technology Department of Sichuan Province ( 2019YFH0103 ), the Fundamental Research Funds for the Central Universities ( 2682020ZT76 ) and the Innovation and Technology Fund ( ITS/065/19 ) from The Hong Kong Innovation and Technology Commission (ITC). We would like to thank Analytical and Testing Center of Southwest Jiaotong University for scanning electron microscope detection.
Publisher Copyright:
© 2020
PY - 2021/4/15
Y1 - 2021/4/15
N2 - Blood-contacting devices have emerged in the 21st century as the go-to clinical treatment for severe cardiovascular diseases. Due to their poor hemocompatibility, many coatings have been developed to improve their biocompatibility with limited success due to the lack of robustness in biomolecule conjugation. In this paper, we propose a new self-protonating, plasma polymerized, superimposed multi-layered nanoreservoir coating for immobilization of charged biomolecules, and demonstrated its high surface charge density, loading capabilities and most importantly, bioactivity retention of biomolecules. Using heparin as a model therapeutic, we demonstrated the advantages of our coating strategy through in vitro and ex vivo means. Our findings will open a new path in clinical device coating strategies in biomolecule functionalization to benefit many patients worldwide.
AB - Blood-contacting devices have emerged in the 21st century as the go-to clinical treatment for severe cardiovascular diseases. Due to their poor hemocompatibility, many coatings have been developed to improve their biocompatibility with limited success due to the lack of robustness in biomolecule conjugation. In this paper, we propose a new self-protonating, plasma polymerized, superimposed multi-layered nanoreservoir coating for immobilization of charged biomolecules, and demonstrated its high surface charge density, loading capabilities and most importantly, bioactivity retention of biomolecules. Using heparin as a model therapeutic, we demonstrated the advantages of our coating strategy through in vitro and ex vivo means. Our findings will open a new path in clinical device coating strategies in biomolecule functionalization to benefit many patients worldwide.
KW - Biomoleculenanoreservoir
KW - Blood contacting surface
KW - Plasma polymerization
KW - Self-protonation
UR - http://www.scopus.com/inward/record.url?scp=85098595981&partnerID=8YFLogxK
U2 - 10.1016/j.cej.2020.128313
DO - 10.1016/j.cej.2020.128313
M3 - Journal article
AN - SCOPUS:85098595981
SN - 1385-8947
VL - 410
JO - Chemical Engineering Journal
JF - Chemical Engineering Journal
M1 - 128313
ER -
