Searching cell-secreted proteomes for potential urinary bladder tumor markers

Chiao Yun Lin, Ke Hung Tsui, Chih Chia Yu, Chun Wei Yeh, Phei Lang Chang, Yat Ming Yung

Research output: Journal article publicationJournal articleAcademic researchpeer-review

34 Citations (Scopus)

Abstract

To search for biomarkers critical for bladder carcinoma diagnosis and prognosis, secreted proteomes of highly malignant U1 and pre-malignant U4 cell lines were initially analyzed. Proteins in the culture media of the U1 and U4 cell lines were systematically examined by SDS-PAGE combined with MALDI-TOF MS. Among them, expression of pro-u-plasminogen activator (pro-u-PA) was confirmed by Western blot analysis and further evaluated. In analyzing urine samples from bladder cancer patients and normal subjects, we established a statistically significant relationship between the low level and absence of pro-u-PA in urine with high stages and grades of the tumor samples. Constitutive expression of Ras dominant negative protein led to increased expression of pro-u-PA in culture media, indicating that the loss of pro-u-PA is associated with oncogenic transformation. Analysis of cancer-secreted proteomes can be a feasible, non-invasive and efficient strategy for searching potential bladder tumor biomarkers. Our work also has identified the loss of pro-u-PA in urine as potential marker of more advanced bladder carcinoma. KGaA.
Original languageEnglish
Pages (from-to)4381-4389
Number of pages9
JournalProteomics
Volume6
Issue number15
DOIs
Publication statusPublished - 1 Aug 2006
Externally publishedYes

Keywords

  • Bladder carcinoma
  • Secreted proteins
  • Urine tumor markers

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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