SARS-CoV-2 infection activates inflammatory macrophages in vascular immune organoids

Chiu Wang Chau; Alex To; Rex K.H. Au-Yeung; Kaiming Tang; Yang Xiang; Degong Ruan; Lanlan Zhang; Hera Wong; Shihui Zhang; Man Ting Au; Seok Chung; Euijeong Song; Dong-Hee Choi; Pentao Liu; Shuofeng Yuan; Chunyi Wen; Ryohichi Sugimura

doi:10.1038/s41598-024-59405-9

SARS-CoV-2 infection activates inflammatory macrophages in vascular immune organoids

Chiu Wang Chau, Alex To, Rex K.H. Au-Yeung, Kaiming Tang, Yang Xiang, Degong Ruan, Lanlan Zhang, Hera Wong, Shihui Zhang, Man Ting Au, Seok Chung, Euijeong Song, Dong-Hee Choi, Pentao Liu, Shuofeng Yuan, Chunyi Wen (Corresponding Author), Ryohichi Sugimura (Corresponding Author)

Department of Biomedical Engineering

Research output: Journal article publication › Journal article › Academic research › peer-review

4 Citations (Scopus)

Abstract

SARS-CoV-2 provokes devastating tissue damage by cytokine release syndrome and leads to multi-organ failure. Modeling the process of immune cell activation and subsequent tissue damage is a significant task. Organoids from human tissues advanced our understanding of SARS-CoV-2 infection mechanisms though, they are missing crucial components: immune cells and endothelial cells. This study aims to generate organoids with these components. We established vascular immune organoids from human pluripotent stem cells and examined the effect of SARS-CoV-2 infection. We demonstrated that infections activated inflammatory macrophages. Notably, the upregulation of interferon signaling supports macrophages’ role in cytokine release syndrome. We propose vascular immune organoids are a useful platform to model and discover factors that ameliorate SARS-CoV-2-mediated cytokine release syndrome.

Original language	English
Journal	Scientific Reports
DOIs	https://doi.org/10.1038/s41598-024-59405-9
Publication status	Published - 16 Apr 2024

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title = "SARS-CoV-2 infection activates inflammatory macrophages in vascular immune organoids",

abstract = "SARS-CoV-2 provokes devastating tissue damage by cytokine release syndrome and leads to multi-organ failure. Modeling the process of immune cell activation and subsequent tissue damage is a significant task. Organoids from human tissues advanced our understanding of SARS-CoV-2 infection mechanisms though, they are missing crucial components: immune cells and endothelial cells. This study aims to generate organoids with these components. We established vascular immune organoids from human pluripotent stem cells and examined the effect of SARS-CoV-2 infection. We demonstrated that infections activated inflammatory macrophages. Notably, the upregulation of interferon signaling supports macrophages{\textquoteright} role in cytokine release syndrome. We propose vascular immune organoids are a useful platform to model and discover factors that ameliorate SARS-CoV-2-mediated cytokine release syndrome.",

author = "Chau, \{Chiu Wang\} and Alex To and Au-Yeung, \{Rex K.H.\} and Kaiming Tang and Yang Xiang and Degong Ruan and Lanlan Zhang and Hera Wong and Shihui Zhang and Au, \{Man Ting\} and Seok Chung and Euijeong Song and Dong-Hee Choi and Pentao Liu and Shuofeng Yuan and Chunyi Wen and Ryohichi Sugimura",

year = "2024",

month = apr,

day = "16",

doi = "10.1038/s41598-024-59405-9",

language = "English",

journal = "Scientific Reports",

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T1 - SARS-CoV-2 infection activates inflammatory macrophages in vascular immune organoids

AU - Chau, Chiu Wang

AU - To, Alex

AU - Au-Yeung, Rex K.H.

AU - Tang, Kaiming

AU - Xiang, Yang

AU - Ruan, Degong

AU - Zhang, Lanlan

AU - Wong, Hera

AU - Zhang, Shihui

AU - Au, Man Ting

AU - Chung, Seok

AU - Song, Euijeong

AU - Choi, Dong-Hee

AU - Liu, Pentao

AU - Yuan, Shuofeng

AU - Wen, Chunyi

AU - Sugimura, Ryohichi

PY - 2024/4/16

Y1 - 2024/4/16

N2 - SARS-CoV-2 provokes devastating tissue damage by cytokine release syndrome and leads to multi-organ failure. Modeling the process of immune cell activation and subsequent tissue damage is a significant task. Organoids from human tissues advanced our understanding of SARS-CoV-2 infection mechanisms though, they are missing crucial components: immune cells and endothelial cells. This study aims to generate organoids with these components. We established vascular immune organoids from human pluripotent stem cells and examined the effect of SARS-CoV-2 infection. We demonstrated that infections activated inflammatory macrophages. Notably, the upregulation of interferon signaling supports macrophages’ role in cytokine release syndrome. We propose vascular immune organoids are a useful platform to model and discover factors that ameliorate SARS-CoV-2-mediated cytokine release syndrome.

AB - SARS-CoV-2 provokes devastating tissue damage by cytokine release syndrome and leads to multi-organ failure. Modeling the process of immune cell activation and subsequent tissue damage is a significant task. Organoids from human tissues advanced our understanding of SARS-CoV-2 infection mechanisms though, they are missing crucial components: immune cells and endothelial cells. This study aims to generate organoids with these components. We established vascular immune organoids from human pluripotent stem cells and examined the effect of SARS-CoV-2 infection. We demonstrated that infections activated inflammatory macrophages. Notably, the upregulation of interferon signaling supports macrophages’ role in cytokine release syndrome. We propose vascular immune organoids are a useful platform to model and discover factors that ameliorate SARS-CoV-2-mediated cytokine release syndrome.

U2 - 10.1038/s41598-024-59405-9

DO - 10.1038/s41598-024-59405-9

M3 - Journal article

SN - 2045-2322

JO - Scientific Reports

JF - Scientific Reports

ER -

SARS-CoV-2 infection activates inflammatory macrophages in vascular immune organoids

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