Roles of paroxetine and corticosterone on adult mammalian ciliary body cell proliferation

Hua Wang, Wui Man Lau, Suk Yu Yau, Suk Yee Li, Nelson Leung, Ning Li Wang, Siu Wa Tang, Tatia Lee, Kwok Fai So

Research output: Journal article publicationJournal articleAcademic researchpeer-review

4 Citations (Scopus)

Abstract

Background The neurogenesis in retina of adult mammals is generally abolished, and this renders the retina lack of regenerative capacity. Despite this, there is a small population of nestin-positive cells in the ciliary epithelium which retains neurogenic potential. The present study aimed at investigating the effect of two drugs, corticosterone and paroxetine, on the cell proliferation of the ciliary body. Methods Adult Sprague-Dawley rats were given vehicle, corticosterone, paroxetine, or both corticosterone and paroxetine treatment for 14 days. Cell proliferation in the ciliary body was quantified using 5-bromo-2-deoxyuridine (BrdU) immunohistochemistry. Co-labelling of BrdU and stem cell marker was used to phenotype the BrdU immunoreactive cells. Results Corticosterone treatment suppressed while paroxetine treatment increased the cell proliferation of the ciliary body. Co-labelling with cell markers revealed that the BrdU positive cells also showed nestin expression but not glial fibrillary acidic protein (GFAP). Conclusions The results illustrate that proliferation of retinal progenitor cells situated in ciliary body are subjected to regulation by selective serotonin reuptake inhibitors (SSRI) and corticosteroid, which is similar to our previous findings in neurogenic regions in central nervous system (CNS). Paroxetine treatment could reverse the suppressive effect of corticosterone on ciliary body cell proliferation. This provides information for future investigation of retinal stem cell biology and potential treatment of retinal degenerative diseases.
Original languageEnglish
Pages (from-to)1305-1310
Number of pages6
JournalChinese Medical Journal
Volume123
Issue number10
DOIs
Publication statusPublished - 1 Jan 2010
Externally publishedYes

Keywords

  • 5-bromo-2-deoxyuridine
  • Cell proliferation
  • Ciliary body
  • Corticosterone
  • Neurogenesis
  • Paroxetine

ASJC Scopus subject areas

  • Medicine(all)

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