Roles of miRNAs in microcystin-LR-induced Sertoli cell toxicity

Yuan Zhou, Hui Wang, Cong Wang, Xuefeng Qiu, Mikael Benson, Xiaoqin Yin, Xiang Zou, Dongmei Li, Xiaodong Han

Research output: Journal article publicationJournal articleAcademic researchpeer-review

18 Citations (Scopus)

Abstract

Microcystin (MC)-LR, a cyclic heptapeptide, is a potent reproductive system toxin. To understand the molecular mechanisms of MC-induced reproductive system cytotoxicity, we evaluated global changes of miRNA and mRNA expression in mouse Sertoli cells following MC-LR treatment. Our results revealed that the exposure to MC-LR resulted in an altered miRNA expression profile that might be responsible for the modulation of mRNA expression. Bio-functional analysis indicated that the altered genes were involved in specific cellular processes, including cell death and proliferation. Target gene analysis suggested that junction injury in Sertoli cells exposed to MC-LR might be mediated by miRNAs through the regulation of the Sertoli cell-Sertoli cell pathway. Collectively, these findings may enhance our understanding on the modes of action of MC-LR on mouse Sertoli cells as well as the molecular mechanisms underlying the toxicity of MC-LR on the male reproductive system.
Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalToxicology and Applied Pharmacology
Volume287
Issue number1
DOIs
Publication statusPublished - 5 Aug 2015
Externally publishedYes

Keywords

  • Bioinformatics
  • Microcystin-LR
  • MicroRNA
  • MRNA
  • Sertoli cell
  • Toxicity

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

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