Role of monoamine oxidases in the exaggerated 5-hydroxytryptamine-induced tension development of human isolated preeclamptic umbilical artery

Sai Wang Seto, Ho Yeung Lam, Wing Sze Lau, Alice L.S. Au, Tsz Yan Lam, Stephen S.C. Chim, Sai Ming Ngai, Shun Wan Chan, Tak Yeung Leung, John H.K. Yeung, Siu Kai Kong, George P.H. Leung, Simon M.Y. Lee, Yiu Wa Kwan

Research output: Journal article publicationJournal articleAcademic researchpeer-review

9 Citations (Scopus)

Abstract

We investigated the role(s) of monoamine oxidases (MAOs) on the altered 5-hydroxytryptamine (5-HT, serotonin)-induced tension development of the isolated umbilical artery of preeclamptic pregnancy of Chinese women. An enhanced 5-HT-induced tension development of the umbilical artery of preeclamptic pregnancy was observed when compared with that of normal pregnancy. The enhanced component of 5-HT-induced tension development was eradicated by clorgyline (a MAO-A inhibitor). Blockade of eNOS (endothelial isoform nitric oxide synthase) (Nω-nitro-l-arginine methyl ester), 5-HT transporter (citalopram), 5-HT receptor subtypes (5HT2B, SB 204741; 5-HT2C, RS 102221; 5-HT7, SB 269970), and endothelium denudation of the umbilical artery of normal pregnancy mimicked the enhanced 5-HT-induced tension development as observed in the preeclamptic tissues. In contrast, no apparent changes in 5-HT-induced tension development of the umbilical artery of preeclamptic pregnancy were observed with the same pharmacological manipulations. A decreased protein expression levels of MAO-A and eNOS (no iNOS and MAO-B expression was detected) and no change in caveolin-1 and 5-HT transporter expression were demonstrated in the umbilical artery (endothelium intact) lysate of preeclamptic pregnancy, compared to that of the umbilical artery of normal pregnancy. Thus, in the umbilical artery of preeclamptic pregnancy, a decrease of MAO-A and eNOS protein expression levels are probably associated with, or responsible for, the exaggerated 5-HT-induced tension development.

Original languageEnglish
Pages (from-to)129-137
Number of pages9
JournalEuropean Journal of Pharmacology
Volume605
Issue number1-3
DOIs
Publication statusPublished - 1 Mar 2009

Keywords

  • 5-hydroxytryptamine
  • Monoamine oxidase
  • Preeclampsia
  • Umbilical artery

ASJC Scopus subject areas

  • Pharmacology

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