Abstract
A Gram-negative bacterium Pseudomonas putida 5-x with high Cu2+accumulating capability was isolated from electroplating effluent in Kwun Tong, Hong Kong. The pretreated cells without superficial layer-capsule, isolated cell envelopes and the separated peptidoglycan layer materials were obtained from fresh P. putida 5-x cells, their Cu2+adsorption capacities and properties were compared with that of the fresh cells. Pretreatment by 0.1 mol L-1HCl enhanced Cu2+adsorption capacity due to the degradation of cell superficial layer-capsule of P. putida 5-x cells. Isolated cell envelopes possessed five times more Cu2+adsorption capacity than that of fresh intact cell. The Cu2+adsorption of separated peptidoglycan layer materials indicated that the peptidoglycan layer only played 10-15% part of the Cu2+adsorption capacity, and implied other cell surface components such as outer membrane or inner membrane might play an important role in such high Cu2+binding of the cell envelopes. The adsorption process of fresh cells, pretreated cells and cell envelopes of P. putida 5-x could be described with Freundlich isotherm, while the adsorption of Cu2+by separated peptidoglycan layer materials was better described with Langmuir isotherm.
| Original language | English |
|---|---|
| Pages (from-to) | 561-568 |
| Number of pages | 8 |
| Journal | Water Research |
| Volume | 37 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1 Jan 2003 |
Keywords
- Adsorption isotherm
- Cell capsule
- Cell envelopes
- Cu adsorption capacity 2+
- Peptidoglycan layer (PEG)
- Pseudomonas putida
ASJC Scopus subject areas
- Ecological Modelling
- Water Science and Technology
- Waste Management and Disposal
- Pollution