Role of cell surface components on Cu2+adsorption by Pseudomonas putida 5-x isolated from electroplating effluent

L. Wang, H. Chua, Q. Zhou, P. K. Wong, S. N. Sin, Wai Hung Lo, P. H. Yu

Research output: Journal article publicationJournal articleAcademic researchpeer-review

24 Citations (Scopus)

Abstract

A Gram-negative bacterium Pseudomonas putida 5-x with high Cu2+accumulating capability was isolated from electroplating effluent in Kwun Tong, Hong Kong. The pretreated cells without superficial layer-capsule, isolated cell envelopes and the separated peptidoglycan layer materials were obtained from fresh P. putida 5-x cells, their Cu2+adsorption capacities and properties were compared with that of the fresh cells. Pretreatment by 0.1 mol L-1HCl enhanced Cu2+adsorption capacity due to the degradation of cell superficial layer-capsule of P. putida 5-x cells. Isolated cell envelopes possessed five times more Cu2+adsorption capacity than that of fresh intact cell. The Cu2+adsorption of separated peptidoglycan layer materials indicated that the peptidoglycan layer only played 10-15% part of the Cu2+adsorption capacity, and implied other cell surface components such as outer membrane or inner membrane might play an important role in such high Cu2+binding of the cell envelopes. The adsorption process of fresh cells, pretreated cells and cell envelopes of P. putida 5-x could be described with Freundlich isotherm, while the adsorption of Cu2+by separated peptidoglycan layer materials was better described with Langmuir isotherm.
Original languageEnglish
Pages (from-to)561-568
Number of pages8
JournalWater Research
Volume37
Issue number3
DOIs
Publication statusPublished - 1 Jan 2003

Keywords

  • Adsorption isotherm
  • Cell capsule
  • Cell envelopes
  • Cu adsorption capacity 2+
  • Peptidoglycan layer (PEG)
  • Pseudomonas putida

ASJC Scopus subject areas

  • Ecological Modelling
  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution

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