Retrograde labeling of retinal ganglion cells by application of fluoro-gold on the surface of superior colliculus

Kin Chiu, Wui Man Lau, Sze chun Yeung, Raymond Chuen Chung Chang, Kwok Fai So

Research output: Journal article publicationJournal articleAcademic researchpeer-review

32 Citations (Scopus)

Abstract

Retinal ganglion cell (RGC) counting is essential to evaluate retinal degeneration especially in glaucoma. Reliable RGC labeling is fundamental for evaluating the effects of any treatment. In rat, about 98% of RGCs is known to project to the contra lateral superior calicles (SC) (Forrester and Peters, 1967). Applying flour-gold (FG) on the surface of SC can label almost all the RGCs, so that we can focus on this most vulnerable retinal neuron in glaucoma. FG is taken up by the axon terminals of retinal ganglion cells and bilaterally transported retrogradely to its somas in the retina. Compare with retrograde labeling of RGC by putting FG at stump of transected optic nerve for 2 days, the interference of RGC survival is minimized. Compare with cresyl violet staining that stains RGCs, amacrine cells and endothelium of the blood vessel in the retinal ganglion cell layer, this labeling method is more specific to the RGC. This video describes the method of retrograde labeling of RGC by applying FG on the surface of SC. The surgical procedures include drilling the skull; aspirating the cortex to expose the SC and applying gelatin sponge over entire dorsal surface of SC are shown. Useful tips for avoiding massive intracranial bleeding and aspiration of the SC have been given.
Original languageEnglish
Article numbere819
JournalJournal of Visualized Experiments
Issue number16
DOIs
Publication statusPublished - 1 Jan 2008
Externally publishedYes

Keywords

  • Experimental glaucoma
  • Issue 16
  • Neuroscience
  • Ophthalmology research
  • Retinal ganglion cells
  • Retrograde labeling
  • Superior colliculus

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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