Resuspension of deposited particles is a pollutant source in indoor environments. Previous studies of resuspension have shown a deviation from actual real life situations and the cleanliness of the surfaces in the studies has been considered as the major factor leading to the deviation. This research studies the detachment behavior of polyethylene (PE) particles on dusty surfaces using a centrifugal method. The dusty surface was produced by settling Arizona test dust (ATD) on a cleaned Poly(methyl methacrylate) (PMMA) substrate by gravitational settling inside a deposition chamber. It was found that the magnitude of force required to remove the target PE particles from the dusty surface decreased significantly compared to that on the cleaned substrate. Also, the removal force decreased linearly with the increase of dust density.