Abstract
Structured illumination can be employed to extend the lateral resolution of wide-field fluorescence microscopy. Since a structured illumination microscopy image is reconstructed from a series of several acquired images, we develop a modified formulation of the imaging response of the microscope and a probabilistic analysis to assess the resolution performance. We use this model to compare the fluorescence imaging performance of structured illumination techniques to confocal microscopy. Specifically, we examine the trade-off between achievable lateral resolution and signal-to-noise ratio when photon shot noise is dominant. We conclude that for a given photon budget, structured illumination invariably achieves better lateral resolution than confocal microscopy.
| Original language | English |
|---|---|
| Pages (from-to) | 1319-1329 |
| Number of pages | 11 |
| Journal | Journal of the Optical Society of America A: Optics and Image Science, and Vision |
| Volume | 25 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1 Jan 2008 |
| Externally published | Yes |
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Computer Vision and Pattern Recognition