TY - JOUR
T1 - Remote Control of Heterodimeric Magnetic Nanoswitch Regulates the Adhesion and Differentiation of Stem Cells
AU - Kang, Heemin
AU - Jung, Hee Joon
AU - Wong, Dexter Siu Hong
AU - Kim, Sung Kyu
AU - Lin, Sien
AU - Chan, Kai Fung
AU - Zhang, Li
AU - Li, Gang
AU - Dravid, Vinayak P.
AU - Bian, Liming
PY - 2018/5/9
Y1 - 2018/5/9
N2 - Remote, noninvasive, and reversible control over the nanoscale presentation of bioactive ligands, such as Arg-Gly-Asp (RGD) peptide, is highly desirable for temporally regulating cellular functions in vivo. Herein, we present a novel strategy for physically uncaging RGD using a magnetic field that allows safe and deep tissue penetration. We developed a heterodimeric nanoswitch consisting of a magnetic nanocage (MNC) coupled to an underlying RGD-coated gold nanoparticle (AuNP) via a long flexible linker. Magnetically controlled movement of MNC relative to AuNP allowed reversible uncaging and caging of RGD that modulate physical accessibility of RGD for integrin binding, thereby regulating stem cell adhesion, both in vitro and in vivo. Reversible RGD uncaging by the magnetic nanoswitch allowed temporal regulation of stem cell adhesion, differentiation, and mechanosensing. This physical and reversible RGD uncaging utilizing heterodimeric magnetic nanoswitch is unprecedented and holds promise in the remote control of cellular behaviors in vivo.
AB - Remote, noninvasive, and reversible control over the nanoscale presentation of bioactive ligands, such as Arg-Gly-Asp (RGD) peptide, is highly desirable for temporally regulating cellular functions in vivo. Herein, we present a novel strategy for physically uncaging RGD using a magnetic field that allows safe and deep tissue penetration. We developed a heterodimeric nanoswitch consisting of a magnetic nanocage (MNC) coupled to an underlying RGD-coated gold nanoparticle (AuNP) via a long flexible linker. Magnetically controlled movement of MNC relative to AuNP allowed reversible uncaging and caging of RGD that modulate physical accessibility of RGD for integrin binding, thereby regulating stem cell adhesion, both in vitro and in vivo. Reversible RGD uncaging by the magnetic nanoswitch allowed temporal regulation of stem cell adhesion, differentiation, and mechanosensing. This physical and reversible RGD uncaging utilizing heterodimeric magnetic nanoswitch is unprecedented and holds promise in the remote control of cellular behaviors in vivo.
UR - http://www.scopus.com/inward/record.url?scp=85046413596&partnerID=8YFLogxK
U2 - 10.1021/jacs.8b03001
DO - 10.1021/jacs.8b03001
M3 - Journal article
C2 - 29681155
AN - SCOPUS:85046413596
SN - 0002-7863
VL - 140
SP - 5909
EP - 5913
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 18
ER -