Release monitoring of single cells on a microfluidic device coupled with fluorescence microscopy and electrochemistry

B.X. Shi, Y. Wang, T.L. Lam, W.H. Huang, K. Zhang, Yun Chung Leung, H.L.W. Chan

Research output: Journal article publicationJournal articleAcademic researchpeer-review

Abstract

A method for monitoring the biological exocytotic phenomena on a microfluidic system was proposed. A microfluidic device coupled with functionalities of fluorescence imaging and amperometric detection has been developed to enable the real-time monitoring of the exocytotic events. Exocytotic release of single SH-SY5Y neuroblastoma cells was studied. By staining the cells located on integrated microelectrodes with naphthalene-2,3-dicarboxaldehyde, punctuate fluorescence consistent with localization of neurotransmitters stored in vesicles was obtained. The stimulated exocytotic release was successfully observed at the surface of SH-SY5Y cells without refitting the commercial inverted fluorescence microscope. Spatially and temporally resolved exocytotic events from single cells on a microfluidic device were visualized in real time using fluorescence microscopy and were amperometrically recorded by the electrochemical system simultaneously. This coupled technique is simple and is hoped to provide new insights into the mechanisms responsible for the kinetics of exocytosis.
Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalBiomicrofluidics
Volume4
Issue number4
DOIs
Publication statusPublished - 30 Dec 2010

Keywords

  • Amperometric sensors
  • Biochemistry
  • BioMEMS
  • Cellular biophysics
  • Electrochemistry
  • Fluorescence
  • Microfluidics
  • Neurophysiology
  • Optical microscopy
  • Toxicology

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Physical and Theoretical Chemistry
  • Materials Science(all)
  • Condensed Matter Physics

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