Regulation of lipid droplets in live preadipocytes using optical diffraction tomography and Raman spectroscopy

Chao Mao Hsieh; Patricia Yang Liu; Lip Ket Chin; Jing Bo Zhang; Kuan Wang; Kung Bin Sung; Wee Ser; Tarik Bourouina; Yamin Leprince-Wang; Ai Qun Liu

doi:10.1364/OE.27.022994

Regulation of lipid droplets in live preadipocytes using optical diffraction tomography and Raman spectroscopy

Chao Mao Hsieh, Patricia Yang Liu, Lip Ket Chin, Jing Bo Zhang, Kuan Wang, Kung Bin Sung, Wee Ser, Tarik Bourouina, Yamin Leprince-Wang, Ai Qun Liu

Research output: Journal article publication › Journal article › Academic research › peer-review

9 Citations (Scopus)

Abstract

Lipid droplets have gained strong interest in recent years to comprehend how they function and coordinate with other parts of the cell. However, it remains challenging to study the regulation of lipid droplets in live preadipocytes using conventional microscopic techniques. In this paper, we study the effects of fatty acid stimulation and cell starvation on lipid droplets using optical diffraction tomography and Raman spectroscopy by measuring size, refractive index, volume, dry mass and degree of unsaturation. The increase of fatty acids causes an increase in the number and dry mass of lipid droplets. During starvation, the number of lipid droplets increases drastically, which are released to mitochondria to release energy. Studying lipid droplets under different chemical stimulations could help us understand the regulation of lipid droplets for metabolic disorders, such as obesity and diabetes.

Original language	English
Pages (from-to)	22994-23008
Number of pages	15
Journal	Optics Express
Volume	27
Issue number	16
DOIs	https://doi.org/10.1364/OE.27.022994
Publication status	Published - 5 Aug 2019
Externally published	Yes

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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10.1364/OE.27.022994

Cite this

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title = "Regulation of lipid droplets in live preadipocytes using optical diffraction tomography and Raman spectroscopy",

abstract = "Lipid droplets have gained strong interest in recent years to comprehend how they function and coordinate with other parts of the cell. However, it remains challenging to study the regulation of lipid droplets in live preadipocytes using conventional microscopic techniques. In this paper, we study the effects of fatty acid stimulation and cell starvation on lipid droplets using optical diffraction tomography and Raman spectroscopy by measuring size, refractive index, volume, dry mass and degree of unsaturation. The increase of fatty acids causes an increase in the number and dry mass of lipid droplets. During starvation, the number of lipid droplets increases drastically, which are released to mitochondria to release energy. Studying lipid droplets under different chemical stimulations could help us understand the regulation of lipid droplets for metabolic disorders, such as obesity and diabetes.",

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AU - Hsieh, Chao Mao

AU - Liu, Patricia Yang

AU - Chin, Lip Ket

AU - Zhang, Jing Bo

AU - Wang, Kuan

AU - Sung, Kung Bin

AU - Ser, Wee

AU - Bourouina, Tarik

AU - Leprince-Wang, Yamin

AU - Liu, Ai Qun

PY - 2019/8/5

Y1 - 2019/8/5

N2 - Lipid droplets have gained strong interest in recent years to comprehend how they function and coordinate with other parts of the cell. However, it remains challenging to study the regulation of lipid droplets in live preadipocytes using conventional microscopic techniques. In this paper, we study the effects of fatty acid stimulation and cell starvation on lipid droplets using optical diffraction tomography and Raman spectroscopy by measuring size, refractive index, volume, dry mass and degree of unsaturation. The increase of fatty acids causes an increase in the number and dry mass of lipid droplets. During starvation, the number of lipid droplets increases drastically, which are released to mitochondria to release energy. Studying lipid droplets under different chemical stimulations could help us understand the regulation of lipid droplets for metabolic disorders, such as obesity and diabetes.

AB - Lipid droplets have gained strong interest in recent years to comprehend how they function and coordinate with other parts of the cell. However, it remains challenging to study the regulation of lipid droplets in live preadipocytes using conventional microscopic techniques. In this paper, we study the effects of fatty acid stimulation and cell starvation on lipid droplets using optical diffraction tomography and Raman spectroscopy by measuring size, refractive index, volume, dry mass and degree of unsaturation. The increase of fatty acids causes an increase in the number and dry mass of lipid droplets. During starvation, the number of lipid droplets increases drastically, which are released to mitochondria to release energy. Studying lipid droplets under different chemical stimulations could help us understand the regulation of lipid droplets for metabolic disorders, such as obesity and diabetes.

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Regulation of lipid droplets in live preadipocytes using optical diffraction tomography and Raman spectroscopy

Abstract

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