Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system

Wee-Keong Vincent Keng, Kojiro Yae, Tomoko Hayakawa, Sumi Mizuno, Yoshihiro Uno, Kosuke Yusa, Chikara Kokubu, Taroh Kinoshita, Keiko Akagi, Nancy A. Jenkins, Neal G. Copeland, Kyoji Horie, Junji Takeda

Research output: Journal article publicationJournal articleAcademic researchpeer-review

96 Citations (Scopus)

Abstract

Recent consolidation of the whole-genome sequence with genome-wide transcriptome profiling revealed the existence of functional units within the genome in specific chromosomal regions, as seen in the coordinated expression of gene clusters and colocalization of functionally related genes. An efficient region-specific mutagenesis screen would greatly facilitate research in addressing the importance of these clusters. Here we use the 'local hopping' phenomenon of a DNA-type transposon, Sleeping Beauty (SB), for region-specific saturation mutagenesis. A transgenic mouse containing both transposon (acts as a mutagen) and transposase (recognizes and mobilizes the transposon) was bred for germ-cell transposition events, allowing us to generate many mutant mice. All genes within a 4-Mb region of the original donor site were mutated by SB, indicating the potential of this system for functional genomic studies within a specific chromosomal region.
Original languageEnglish
Pages (from-to)763-769
Number of pages7
JournalNature Methods
Volume2
Issue number10
DOIs
Publication statusPublished - 1 Oct 2005
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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