Reelin is a platelet protein and functions as a positive regulator of platelet spreading on fibrinogen

Wei Lien Tseng, Chien-Ling Huang, Kowit Yu Chong, Chang Huei Liao, Arnold Stern, Ju Chien Cheng, Ching Ping Tseng

Research output: Journal article publicationJournal articleAcademic researchpeer-review

16 Citations (Scopus)

Abstract

Abnormalities of platelet functions have been linked to reelin-impaired neuronal disorders. However, little attention has been given to understanding the interplay between reelin and platelet. In this study, reelin was found to present in the human platelets and megakaryocyte-like leukemic cells. Reelin-binding assays revealed that extracellular reelin can interact with platelets through the receptor belonging to the low density lipoprotein receptor gene family. The reelin-to-platelet interactions enhance platelet spreading on fibrinogen concomitant with the augmentation of lamellipodia formation and F-actin bundling. In contrast, reelin has no effect on integrin IIb3 activation and agonist-induced platelet aggregation. Molecular analysis revealed that the up-regulation of Rac1 activity and the inhibition of protein kinase C-Thr505 phosphorylation are important for reelin-mediated enhancement of platelet spreading on fibrinogen. These findings demonstrate for the first time that reelin is present in platelets and the reelin-to-platelet interactions play a novel role in platelet signaling and functions.
Original languageEnglish
Pages (from-to)641-653
Number of pages13
JournalCellular and Molecular Life Sciences
Volume67
Issue number4
DOIs
Publication statusPublished - 1 Feb 2010
Externally publishedYes

Keywords

  • Fibrinogen
  • PKC
  • Platelet spreading
  • Rac1
  • Reelin

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Molecular Medicine
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Medicine(all)

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