ReDuNing, a patented Chinese medicine, reduces the susceptibility to H1N1 influenza of mice loaded with restraint stress

Lu Ping Tang, Zhong Fu Mao, Xiao Xiao Li, Min Chen, Shan Bing Li, Bun Tsoi, Ling Fang Cao, Ling Li, Ji Mei Zeng, Zhi Wei Wang, Gui Yuan Yu, Wei Xiao, Yi Fang Li, Hiroshi Kurihara, Rong Rong He

Research output: Journal article publicationJournal articleAcademic researchpeer-review

26 Citations (Scopus)

Abstract

Introduction: ReDuNing (RDN), a patented traditional antipyretic-detoxicate Chinese medicine, has been widely applied as an anti-inflammatory and anti-infectious drug in Chinese clinics. However, relative experimental data on anti-influenza virus and its subsequent viral pneumonia by RDN are limited. In this study, restraint stress mice model, which could mimic the physiological conditions of the population susceptible to influenza infection, was applied to investigate the protective effect of RDN against influenza. Methods: Mice were infected with H1N1 influenza virus 3 days after restraint, and RDN was administered to mice the day after stress by intraperitoneal injection for 7 days. Body weight and mortality were recorded every day. Histopathologic changes, lung index, nucleoprotein (NP) gene expressions and inflammatory markers in the lungs were also measured. Results: RDN administration significantly decreased lung index, ameliorated lung histopathologic damage and decreased lung NP gene expression in influenza-infected mice loaded with restraint stress. It also decreased phosphorylated inhibitor of nuclear factor kappa B (p-IκB), nuclear factor-kappa B (NF-κB) protein level and interleukin-1β (IL-1β) mRNA expression, increased interferon inducible transmembrane 3 (IFITM3) and mitochondrial antiviral signaling (MAVS) gene expression. Conclusions: This study provided preliminary support to suggest that RDN can reduce the susceptibility to, and the severity of influenza in restraint-stressed mice. The study also supported the potential of implementing RDN as an antiviral and its impact as potential anti-influenza treatments which rebalances homeostasis in the body caused by stress.

Original languageEnglish
Pages (from-to)637-645
Number of pages9
JournalEuropean Journal of Integrative Medicine
Volume6
Issue number6
DOIs
Publication statusPublished - 1 Dec 2014
Externally publishedYes

Keywords

  • Influenza virus
  • Pneumonia
  • ReDuNing
  • Restraint stress

ASJC Scopus subject areas

  • Complementary and alternative medicine

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