Real-time optical biosensor based on differential phase measurement of surface plasmon resonance

H. P. Ho; Wing Cheung Law; S. Y. Wu; Chinlon Lin; S. K. Kong

doi:10.1016/j.bios.2004.09.011

Real-time optical biosensor based on differential phase measurement of surface plasmon resonance

H. P. Ho, Wing Cheung Law, S. Y. Wu, Chinlon Lin, S. K. Kong

Research output: Journal article publication › Journal article › Academic research › peer-review

40 Citations (Scopus)

Abstract

We report a real-time differential phase measurement technique which can be implemented in optical surface plasmon resonance biosensors. The important feature of our design is that sensitivity has been greatly improved by measuring the differential phase change between the s and p-polarizations. Real-time measurement capability is achieved by using a phase extracting routine which continuously monitors the waveforms captured by two photo-detectors. Measurement capability of our setup is demonstrated through real-time monitoring of bovine serum albumin (BSA)/anti-BSA binding reaction. The estimated sensitivity of our current setup is 7.4 ng/ml.

Original language	English
Pages (from-to)	2177-2180
Number of pages	4
Journal	Biosensors and Bioelectronics
Volume	20
Issue number	10 SPEC. ISS.
DOIs	https://doi.org/10.1016/j.bios.2004.09.011
Publication status	Published - 15 Apr 2005
Externally published	Yes

Keywords

Optical phase
Surface plasmon resonance

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Electrochemistry

More information

10.1016/j.bios.2004.09.011

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abstract = "We report a real-time differential phase measurement technique which can be implemented in optical surface plasmon resonance biosensors. The important feature of our design is that sensitivity has been greatly improved by measuring the differential phase change between the s and p-polarizations. Real-time measurement capability is achieved by using a phase extracting routine which continuously monitors the waveforms captured by two photo-detectors. Measurement capability of our setup is demonstrated through real-time monitoring of bovine serum albumin (BSA)/anti-BSA binding reaction. The estimated sensitivity of our current setup is 7.4 ng/ml.",

keywords = "Optical phase, Surface plasmon resonance",

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AU - Ho, H. P.

AU - Law, Wing Cheung

AU - Wu, S. Y.

AU - Lin, Chinlon

AU - Kong, S. K.

PY - 2005/4/15

Y1 - 2005/4/15

N2 - We report a real-time differential phase measurement technique which can be implemented in optical surface plasmon resonance biosensors. The important feature of our design is that sensitivity has been greatly improved by measuring the differential phase change between the s and p-polarizations. Real-time measurement capability is achieved by using a phase extracting routine which continuously monitors the waveforms captured by two photo-detectors. Measurement capability of our setup is demonstrated through real-time monitoring of bovine serum albumin (BSA)/anti-BSA binding reaction. The estimated sensitivity of our current setup is 7.4 ng/ml.

AB - We report a real-time differential phase measurement technique which can be implemented in optical surface plasmon resonance biosensors. The important feature of our design is that sensitivity has been greatly improved by measuring the differential phase change between the s and p-polarizations. Real-time measurement capability is achieved by using a phase extracting routine which continuously monitors the waveforms captured by two photo-detectors. Measurement capability of our setup is demonstrated through real-time monitoring of bovine serum albumin (BSA)/anti-BSA binding reaction. The estimated sensitivity of our current setup is 7.4 ng/ml.

KW - Optical phase

KW - Surface plasmon resonance

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DO - 10.1016/j.bios.2004.09.011

M3 - Journal article

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VL - 20

SP - 2177

EP - 2180

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 10 SPEC. ISS.

ER -

Real-time optical biosensor based on differential phase measurement of surface plasmon resonance

Abstract

Keywords

ASJC Scopus subject areas

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