Real-time intraoperative fluorescence imaging system using light-absorption correction

George Themelis, Jung Sun Yoo, Kwang Sup Soh, Ralf Schulz, Vasilis Ntziachristos

Research output: Journal article publicationJournal articleAcademic researchpeer-review

145 Citations (Scopus)


We present a novel fluorescence imaging system developed for real-time interventional imaging applications. The system implements a correction scheme that improves the accuracy of epiillumination fluorescence images for light intensity variation in tissues. The implementation is based on the use of three cameras operating in parallel, utilizing a common lens, which allows for the concurrent collection of color, fluorescence, and light attenuation images at the excitation wavelength from the same field of view. The correction is based on a ratio approach of fluorescence over light attenuation images. Color images and video is used for surgical guidance and for registration with the corrected fluorescence images. We showcase the performance metrics of this system on phantoms and animals, and discuss the advantages over conventional epi-illumination systems developed for real-time applications and the limits of validity of corrected epi-illumination fluorescence imaging.
Original languageEnglish
Article number064012
JournalJournal of Biomedical Optics
Issue number6
Publication statusPublished - 1 Dec 2009
Externally publishedYes


  • Epi-illumination
  • Fluorescence imaging
  • Intraoperative imaging
  • Light absorption correction
  • Light attenuation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering


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