Rapid production of gene replacement constructs and generation of a green fluorescent protein-tagged centromeric marker in Aspergillus nidulans

Lin Yang; Leena Ukil; Aysha Osmani; Francis Nahm; Jonathan Davies; Colin P.C. De Souza; Xiaowei Dou; Ariadna Perez-Balaguer; Stephen A. Osmani

doi:10.1128/EC.3.5.1359-1362.2004

Rapid production of gene replacement constructs and generation of a green fluorescent protein-tagged centromeric marker in Aspergillus nidulans

Lin Yang, Leena Ukil, Aysha Osmani, Francis Nahm, Jonathan Davies, Colin P.C. De Souza, Xiaowei Dou, Ariadna Perez-Balaguer, Stephen A. Osmani

Research output: Journal article publication › Journal article › Academic research › peer-review

222 Citations (Scopus)

Abstract

A method to rapidly generate gene replacement constructs by fusion PCR is described for Aspergillus nidulans. The utility of the approach is demonstrated by green fluorescent protein (GFP) tagging of A. nidulans ndc80 to visualize centromeres through the cell cycle. The methodology makes possible large-scale GFP tagging, promoter swapping, and deletion analysis of A. nidulans.

Original language	English
Pages (from-to)	1359-1362
Number of pages	4
Journal	Eukaryotic Cell
Volume	3
Issue number	5
DOIs	https://doi.org/10.1128/EC.3.5.1359-1362.2004
Publication status	Published - 1 Oct 2004
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Molecular Biology

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10.1128/EC.3.5.1359-1362.2004

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AU - Nahm, Francis

AU - Davies, Jonathan

AU - De Souza, Colin P.C.

AU - Dou, Xiaowei

AU - Perez-Balaguer, Ariadna

AU - Osmani, Stephen A.

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AB - A method to rapidly generate gene replacement constructs by fusion PCR is described for Aspergillus nidulans. The utility of the approach is demonstrated by green fluorescent protein (GFP) tagging of A. nidulans ndc80 to visualize centromeres through the cell cycle. The methodology makes possible large-scale GFP tagging, promoter swapping, and deletion analysis of A. nidulans.

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Rapid production of gene replacement constructs and generation of a green fluorescent protein-tagged centromeric marker in Aspergillus nidulans

Abstract

ASJC Scopus subject areas

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