Rapid production of gene replacement constructs and generation of a green fluorescent protein-tagged centromeric marker in Aspergillus nidulans

Lin Yang, Leena Ukil, Aysha Osmani, Francis Nahm, Jonathan Davies, Colin P.C. De Souza, Xiaowei Dou, Ariadna Perez-Balaguer, Stephen A. Osmani

Research output: Journal article publicationJournal articleAcademic researchpeer-review

208 Citations (Scopus)


A method to rapidly generate gene replacement constructs by fusion PCR is described for Aspergillus nidulans. The utility of the approach is demonstrated by green fluorescent protein (GFP) tagging of A. nidulans ndc80 to visualize centromeres through the cell cycle. The methodology makes possible large-scale GFP tagging, promoter swapping, and deletion analysis of A. nidulans.
Original languageEnglish
Pages (from-to)1359-1362
Number of pages4
JournalEukaryotic Cell
Issue number5
Publication statusPublished - 1 Oct 2004
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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