Rapid differentiation of Ganoderma species by direct ionization mass spectrometry

Ho Yi Wong, Melody Yee Man Wong, Bin Hu, Pui Kin So, Chi On Chan, Kam Wah Mok, Zhongping Yao

Research output: Journal article publicationJournal articleAcademic researchpeer-review

11 Citations (Scopus)


In this study, direct ionization mass spectrometry (DI-MS) has been developed for rapid differentiation of Ganoderma (known as Lingzhi in Chinese), a very popular and valuable herbal medicine. Characteristic mass spectra can be generated by DI-MS directly from the raw herbal medicines with the application of a high voltage and solvents. Rapid differentiation of the Ganoderma species that are officially stated in the Chinese pharmacopoeia from easily confused Ganoderma species could be achieved based on this method, as the acquired DI-MS spectra showed that ganoderic acids, the major active components of Ganoderma, could be found only in the official Ganoderma species but not in the confused Ganoderma species. In addition, classification of wild and cultivated Ganoderma and potential differentiation of Ganoderma from different geographical locations could be accomplished based on principal component analysis (PCA) or hierarchical clustering analysis (HCA). The method is rapid, simple and reproducible, and can be further extended to analysis of other herbal medicines.
Original languageEnglish
Pages (from-to)99-106
Number of pages8
JournalAnalytica Chimica Acta
Publication statusPublished - 25 Jan 2018


  • Active components
  • Differentiation
  • Direct ionization
  • Ganoderma
  • Mass spectrometry

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy


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