Rapid bacteriophage detection via host cell amplication in a droplet-based optofluidic system

J. Q. Yu, W. Huang, L. K. Chin, A. Q. Liu

Research output: Chapter in book / Conference proceedingConference article published in proceeding or bookAcademic researchpeer-review

Abstract

This paper presents a real-time and label-free bacteriophage detection technique with high sensitivity and low cost by using a droplet-based optofluidic system. The bacteriophages and host cells (E. coli) mixture are encapsulated in microdroplets during their formation in mineral oil. The scattering patterns of the bacteriophages and the host cells containing droplets are captured by an optical sensor and analyzed by a signal processing system. The scattering patterns of microdroplets with different host cell concentrations are measured. The optofluidic system is developed to effectively detect and quantify bacteriophages in real-time with a detection resolution of 10 3. A higher resolution can be realized by using a data processing system to analyze the scattering pattern, which will be done in our future work.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1768-1770
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - Nov 2012
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 28 Oct 20121 Nov 2012

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Conference

Conference16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Country/TerritoryJapan
CityOkinawa
Period28/10/121/11/12

Keywords

  • Bacteriophage
  • Optofluidics
  • Scattering pattern

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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