Rapid and sensitive detection of bacteria response to antibiotics using nanoporous membrane and graphene quantum dot (GQDs)-based electrochemical biosensors

Weiwei Ye, Jiubiao Guo, Xianfeng Bao, Tian Chen, Wenchuan Weng, Sheng Chen, Mo Yang

Research output: Journal article publicationJournal articleAcademic researchpeer-review

16 Citations (Scopus)

Abstract

The wide abuse of antibiotics has accelerated bacterial multiresistance, which means there is a need to develop tools for rapid detection and characterization of bacterial response to antibiotics in the management of infections. In the study, an electrochemical biosensor based on nanoporous alumina membrane and graphene quantum dots (GQDs) was developed for bacterial response to antibiotics detection. Anti-Salmonella antibody was conjugated with amino-modified GQDs by glutaraldehyde and immobilized on silanized nanoporous alumina membranes for Salmonella bacteria capture. The impedance signals across nanoporous membranes could monitor the capture of bacteria on nanoporous membranes as well as bacterial response to antibiotics. This nanoporous membrane and GQD-based electrochemical biosensor achieved rapid detection of bacterial response to antibiotics within 30 min, and the detection limit could reach the pM level. It was capable of investigating the response of bacteria exposed to antibiotics much more rapidly and conveniently than traditional tools. The capability of studying the dynamic effects of antibiotics on bacteria has potential applications in the field of monitoring disease therapy, detecting comprehensive food safety hazards and even life in hostile environment.
Original languageEnglish
Article number603
JournalMaterials
Volume10
Issue number6
DOIs
Publication statusPublished - 31 May 2017

Keywords

  • Bacterial response to antibiotics
  • Electrochemical biosensor
  • Graphene quantum dots
  • Nanoporous alumina membrane
  • Rapid
  • Sensitive

ASJC Scopus subject areas

  • Materials Science(all)

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