Rapid analysis of raw solution samples by C18 pipette-tip electrospray ionization mass spectrometry

Haixing Wang, Pui Kin So, Tsz Tsun Ng, Zhongping Yao

Research output: Journal article publicationJournal articleAcademic researchpeer-review

14 Citations (Scopus)


A C18 pipette-tip electrospray ionization mass spectrometry technique was developed for rapid analysis of raw solution samples. In this technique, a C18 pipette tip was employed for rapid purification and enrichment of analytes in raw sample solutions. The adsorbed analytes were eluted by solvents supplied by a syringe and a syringe pump, and a high voltage was applied onto the syringe needle to induce electrospray ionization at the pipette tip end for mass spectrometric analysis. This technique is simple, easy to assemble, enables generation of stable and reproducible signals, and can be conveniently used for qualitative and quantitative analysis of raw solution samples. Analysis by the technique only involved simple sample preparation procedures followed by direct mass spectrometric detection, all of which could be completed within minutes, while the analytical performances of the technique, including the limit of detection, limit of quantitation, liner range, accuracy and precision, were comparable to those by conventional methods.
Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalAnalytica Chimica Acta
Publication statusPublished - 24 Sep 2014


  • C18
  • Electrospray ionization mass spectrometry
  • Ketamine
  • Pipette tips
  • Proteins
  • Raw solutions

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry
  • Medicine(all)

Cite this