Quantification of Plasma β-Catenin mRNA in Colorectal Cancer and Adenoma Patients

Sze Chuen Cesar Wong, Siu Fong Elena Lo, Moon Tong Cheung, Kai On Enders Ng, Chun Wah Tse, Bo San Paul Lai, King Chung Lee, Y. M Dennis Lo

Research output: Journal article publicationJournal articleAcademic researchpeer-review

87 Citations (Scopus)

Abstract

Purpose: Colorectal cancer is an important cause of cancer deaths. Here, we focused our investigation on the β-catenin gene which is implicated in colorectal carcinogenesis and tested whether β-catenin mRNA is detectable in the plasma of colorectal carcinoma and adenoma patients using quantitative reverse transcriptase-PCR. Experimental Design: Plasma β-catenin mRNA was measured from 58 colorectal carcinoma patients, 49 colorectal adenoma patients, and 43 apparently normal subjects using intron-spanning primers and Taqman probes. Five clinicopathological parameters were studied and correlated with plasma β-catenin mRNA concentration. Additionally, 19 colorectal carcinoma patients after tumor removal were also recruited for plasma β-catenin mRNA measurement to further demonstrate the clinical usefulness of this test. Results: β-catenin mRNA was detected with median concentrations of 8737 (range: 1480-933,100), 1218 (range: 541-2,254) and 291 (range: 0-1,366) copies/mi plasma in colorectal carcinoma, colorectal adenoma, and apparently normal subjects, respectively. Statistical analysis demonstrated that plasma β-catenin mRNA concentration was correlated to tumor stage but not sex, age, lymph node status, and degree in differentiation. Moreover, plasma β-catenin mRNA concentration decreased significantly after tumor removal in 16 of 19 (84%) colorectal carcinoma patients. Conclusions: We conclude that plasma β-catenin mRNA may potentially serve as a marker for colorectal cancer.
Original languageEnglish
Pages (from-to)1613-1617
Number of pages5
JournalClinical Cancer Research
Volume10
Issue number5
DOIs
Publication statusPublished - 1 Mar 2004
Externally publishedYes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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