Qualitative and quantitative analysis of chemical constituents of Centipeda minima by HPLC-QTOF-MS & HPLC-DAD

Chi On Chan, Deng Ping Jin, Nai Ping Dong, Si Bao Chen, Daniel Kam Wah Mok

Research output: Journal article publicationJournal articleAcademic researchpeer-review

30 Citations (Scopus)


A high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS) method in both positive and negative ion modes was established to investigate the major constituents in the ethanolic extract of Centipeda minima (EBSC). Twelve common components including flavones and their glycosides, phenolic and polyphenolic acids, and sesquiterpene lactone were identified in ten batches of samples based on comparison with the retention time and accurate mass of external standards (mass accuracy within 3 ppm) or the fragmentation patterns of tandem MS. Meanwhile, a simple, accurate and reliable HPLC-DAD method was also developed to determine the content of 10 chemical markers simultaneously. Results obtained from method validations including linearity, accuracy and precision showed that this new method is reliable and robust. Isochlorogenic acid A and brevilin A were found to be the most abundant in the ethanol extract of EBSC and could be served as markers for quality control of EBSC.
Original languageEnglish
Pages (from-to)400-407
Number of pages8
JournalJournal of Pharmaceutical and Biomedical Analysis
Publication statusPublished - 5 Jun 2016


  • Brevilin A
  • Centipeda minima
  • Herbal analysis
  • Isochlorogenic acid A
  • Quadrupole time-of-flight mass spectrometry (QTOF MS)
  • Quality control

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry


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