Pulsatile shear stress and high glucose concentrations induced cell death in endothelial cells

J. Q. Yu; L. K. Chin; A. Q. Liu; K. Q. Luo

Pulsatile shear stress and high glucose concentrations induced cell death in endothelial cells

J. Q. Yu
, L. K. Chin
, A. Q. Liu
, K. Q. Luo

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

Abstract

This paper presents the exposure of endothelial cells to high glucose concentration combined with shear stress variety, which mimicking the heart beat cycle stimulates reactive oxygen species (ROS) overproduction and mitochondria damage, and further leads to increased cellular apoptosis. The apoptosis and necroses level is studied using real-time fluorescence microscopy with the measurement of yo-pro-1 iodide and propidium iodide dye. Results show the apoptosis rate matched well with the ROS overproduction and mitochondria damage rate and the study will further unclose the role of diabetes as a contributor to endothelial dysfunction and cardiovascular diseases.

Original language	English
Title of host publication	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages	837-839
Number of pages	3
Publication status	Published - Oct 2011
Externally published	Yes
Event	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States Duration: 2 Oct 2011 → 6 Oct 2011

Publication series

Name	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume	2

Conference

Conference	15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Country/Territory	United States
City	Seattle, WA
Period	2/10/11 → 6/10/11

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cell death
Endothelial cell
Hemodynamic lab-on-a-chip system

ASJC Scopus subject areas

Control and Systems Engineering

Cite this

Yu, J. Q., Chin, L. K., Liu, A. Q., & Luo, K. Q. (2011). Pulsatile shear stress and high glucose concentrations induced cell death in endothelial cells. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 837-839). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).

Yu, J. Q. ; Chin, L. K. ; Liu, A. Q. et al. / Pulsatile shear stress and high glucose concentrations induced cell death in endothelial cells. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. pp. 837-839 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).

@inproceedings{18e0c1ad56274e798b2f45d1de5efde6,

title = "Pulsatile shear stress and high glucose concentrations induced cell death in endothelial cells",

abstract = "This paper presents the exposure of endothelial cells to high glucose concentration combined with shear stress variety, which mimicking the heart beat cycle stimulates reactive oxygen species (ROS) overproduction and mitochondria damage, and further leads to increased cellular apoptosis. The apoptosis and necroses level is studied using real-time fluorescence microscopy with the measurement of yo-pro-1 iodide and propidium iodide dye. Results show the apoptosis rate matched well with the ROS overproduction and mitochondria damage rate and the study will further unclose the role of diabetes as a contributor to endothelial dysfunction and cardiovascular diseases.",

keywords = "Cell death, Endothelial cell, Hemodynamic lab-on-a-chip system",

author = "Yu, \{J. Q.\} and Chin, \{L. K.\} and Liu, \{A. Q.\} and Luo, \{K. Q.\}",

year = "2011",

month = oct,

language = "English",

isbn = "9781618395955",

series = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011",

pages = "837--839",

booktitle = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011",

note = "15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 ; Conference date: 02-10-2011 Through 06-10-2011",

}

Yu, JQ, Chin, LK, Liu, AQ & Luo, KQ 2011, Pulsatile shear stress and high glucose concentrations induced cell death in endothelial cells. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, vol. 2, pp. 837-839, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 2/10/11.

Pulsatile shear stress and high glucose concentrations induced cell death in endothelial cells. / Yu, J. Q.; Chin, L. K.; Liu, A. Q. et al.
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 837-839 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).

Research output: Chapter in book / Conference proceeding › Conference article published in proceeding or book › Academic research › peer-review

TY - GEN

T1 - Pulsatile shear stress and high glucose concentrations induced cell death in endothelial cells

AU - Yu, J. Q.

AU - Chin, L. K.

AU - Liu, A. Q.

AU - Luo, K. Q.

PY - 2011/10

Y1 - 2011/10

N2 - This paper presents the exposure of endothelial cells to high glucose concentration combined with shear stress variety, which mimicking the heart beat cycle stimulates reactive oxygen species (ROS) overproduction and mitochondria damage, and further leads to increased cellular apoptosis. The apoptosis and necroses level is studied using real-time fluorescence microscopy with the measurement of yo-pro-1 iodide and propidium iodide dye. Results show the apoptosis rate matched well with the ROS overproduction and mitochondria damage rate and the study will further unclose the role of diabetes as a contributor to endothelial dysfunction and cardiovascular diseases.

AB - This paper presents the exposure of endothelial cells to high glucose concentration combined with shear stress variety, which mimicking the heart beat cycle stimulates reactive oxygen species (ROS) overproduction and mitochondria damage, and further leads to increased cellular apoptosis. The apoptosis and necroses level is studied using real-time fluorescence microscopy with the measurement of yo-pro-1 iodide and propidium iodide dye. Results show the apoptosis rate matched well with the ROS overproduction and mitochondria damage rate and the study will further unclose the role of diabetes as a contributor to endothelial dysfunction and cardiovascular diseases.

KW - Cell death

KW - Endothelial cell

KW - Hemodynamic lab-on-a-chip system

UR - https://www.scopus.com/pages/publications/84883751324

M3 - Conference article published in proceeding or book

AN - SCOPUS:84883751324

SN - 9781618395955

T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011

SP - 837

EP - 839

BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011

T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011

Y2 - 2 October 2011 through 6 October 2011

ER -

Pulsatile shear stress and high glucose concentrations induced cell death in endothelial cells

Abstract

Publication series

Conference

UN SDGs

Keywords

ASJC Scopus subject areas

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