TY - JOUR
T1 - Pseudoephedrine hydrochloride causes hyperactivity in zebrafish via modulation of the serotonin pathway
AU - Zhou, Yini
AU - Li, Tonglaga
AU - Zhou, Shangzi
AU - Xu, Han
AU - Yin, Xiaoyu
AU - Chen, Hao
AU - Ni, Xuan
AU - Bai, Meirong
AU - Ao, Wuliji
AU - Yang, Jingfeng
AU - Ahmed, R. G.
AU - Zhang, Xuefu
AU - Bao, Shuyin
AU - Yu, Jianhua
AU - Kwok, Kevin W.H.
AU - Dong, Wu
N1 - Funding Information:
This work was funded by the Supported by Program for Innovative Research Team in Universities of Inner Mongolia Autonomous Region of China (NMGIRT2216), National Natural Science Foundation of China (8216141082 and 82060672), Natural Science Foundation of Inner Mongolia Autonomous Region of China (2020MS08103 and 2020MS08101); Open Project Program of Inner Mongolia Research Institute of Traditional Mongolian Medicine Engineering Technology, China (MDK2020002), Open project from Key Laboratory of Traditional Mongolian Medicine Research & Development Engineering, State Ethnic Affairs Commission and Ministry of Education (MDK2019069); Open Project Program of Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, China (MDK2018025, MDK2019072, MDK2019074, and MDK 2019077); Startup Project of Doctor scientific research in Inner Mongolia Minzu University of China (BS555 and BS562).
Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022/10
Y1 - 2022/10
N2 - This study aimed to explore behavioral changes of embryonic and larval zebrafish caused by pseudoephedrine hydrochloride (PSE) and its underlying mechanism. Zebrafish embryos were exposed to 0.5 µM, 2 µM, and 8 µM PSE at 4 h post-fertilization (4 hpf) or 22–23 hpf. Mortality, hatching rate, coiling frequency, heart rate, behavior changes, and related gene expression were observed at different developmental stages. PSE below 8 µM did not affect zebrafish mortality, hatching rate, and heart rate compared with the control group. For embryos, PSE caused an increase at 16–32 hpf in zebrafish coiling frequency which could be rescued by serotonin antagonist WAY100635. Similarly, PSE caused an increase in the swimming distance of zebrafish larvae at 120 hpf. PSE also elevated the expression of serotonin (5-HT)-related genes 5-htr1ab and tph2 and dopamine-related gene dbh. Behavioral changes in zebrafish embryos and larvae caused by PSE may be closely associated with increased expression of 5-HT and dopamine-related genes. This may be reflected that the behavioral changes in zebrafish are a possible PSE monitoring indicator.
AB - This study aimed to explore behavioral changes of embryonic and larval zebrafish caused by pseudoephedrine hydrochloride (PSE) and its underlying mechanism. Zebrafish embryos were exposed to 0.5 µM, 2 µM, and 8 µM PSE at 4 h post-fertilization (4 hpf) or 22–23 hpf. Mortality, hatching rate, coiling frequency, heart rate, behavior changes, and related gene expression were observed at different developmental stages. PSE below 8 µM did not affect zebrafish mortality, hatching rate, and heart rate compared with the control group. For embryos, PSE caused an increase at 16–32 hpf in zebrafish coiling frequency which could be rescued by serotonin antagonist WAY100635. Similarly, PSE caused an increase in the swimming distance of zebrafish larvae at 120 hpf. PSE also elevated the expression of serotonin (5-HT)-related genes 5-htr1ab and tph2 and dopamine-related gene dbh. Behavioral changes in zebrafish embryos and larvae caused by PSE may be closely associated with increased expression of 5-HT and dopamine-related genes. This may be reflected that the behavioral changes in zebrafish are a possible PSE monitoring indicator.
KW - Behavioral changes
KW - Dopamine
KW - Pseudoephedrine hydrochloride
KW - Serotonin
KW - Zebrafish embryos
UR - https://www.scopus.com/pages/publications/85135281756
U2 - 10.1007/s11011-022-01042-0
DO - 10.1007/s11011-022-01042-0
M3 - Journal article
C2 - 35907131
AN - SCOPUS:85135281756
SN - 0885-7490
VL - 37
SP - 2559
EP - 2568
JO - Metabolic Brain Disease
JF - Metabolic Brain Disease
IS - 7
ER -