Pseudoephedrine hydrochloride causes hyperactivity in zebrafish via modulation of the serotonin pathway

Yini Zhou; Tonglaga Li; Shangzi Zhou; Han Xu; Xiaoyu Yin; Hao Chen; Xuan Ni; Meirong Bai; Wuliji Ao; Jingfeng Yang; R. G. Ahmed; Xuefu Zhang; Shuyin Bao; Jianhua Yu; Kevin W.H. Kwok; Wu Dong

doi:10.1007/s11011-022-01042-0

Pseudoephedrine hydrochloride causes hyperactivity in zebrafish via modulation of the serotonin pathway

Yini Zhou, Tonglaga Li, Shangzi Zhou, Han Xu, Xiaoyu Yin, Hao Chen, Xuan Ni, Meirong Bai, Wuliji Ao, Jingfeng Yang, R. G. Ahmed, Xuefu Zhang, Shuyin Bao, Jianhua Yu, Kevin W.H. Kwok, Wu Dong

Research output: Journal article publication › Journal article › Academic research › peer-review

Abstract

This study aimed to explore behavioral changes of embryonic and larval zebrafish caused by pseudoephedrine hydrochloride (PSE) and its underlying mechanism. Zebrafish embryos were exposed to 0.5 µM, 2 µM, and 8 µM PSE at 4 h post-fertilization (4 hpf) or 22–23 hpf. Mortality, hatching rate, coiling frequency, heart rate, behavior changes, and related gene expression were observed at different developmental stages. PSE below 8 µM did not affect zebrafish mortality, hatching rate, and heart rate compared with the control group. For embryos, PSE caused an increase at 16–32 hpf in zebrafish coiling frequency which could be rescued by serotonin antagonist WAY100635. Similarly, PSE caused an increase in the swimming distance of zebrafish larvae at 120 hpf. PSE also elevated the expression of serotonin (5-HT)-related genes 5-htr1ab and tph2 and dopamine-related gene dbh. Behavioral changes in zebrafish embryos and larvae caused by PSE may be closely associated with increased expression of 5-HT and dopamine-related genes. This may be reflected that the behavioral changes in zebrafish are a possible PSE monitoring indicator.

Original language	English
Pages (from-to)	2559-2568
Number of pages	10
Journal	Metabolic Brain Disease
Volume	37
Issue number	7
DOIs	https://doi.org/10.1007/s11011-022-01042-0
Publication status	Published - Oct 2022

Keywords

Behavioral changes
Dopamine
Pseudoephedrine hydrochloride
Serotonin
Zebrafish embryos

ASJC Scopus subject areas

Biochemistry
Clinical Neurology
Cellular and Molecular Neuroscience

More information

10.1007/s11011-022-01042-0

Cite this

@article{8e65dd37898e42d5938d0715411e0a5f,

title = "Pseudoephedrine hydrochloride causes hyperactivity in zebrafish via modulation of the serotonin pathway",

abstract = "This study aimed to explore behavioral changes of embryonic and larval zebrafish caused by pseudoephedrine hydrochloride (PSE) and its underlying mechanism. Zebrafish embryos were exposed to 0.5 µM, 2 µM, and 8 µM PSE at 4 h post-fertilization (4 hpf) or 22–23 hpf. Mortality, hatching rate, coiling frequency, heart rate, behavior changes, and related gene expression were observed at different developmental stages. PSE below 8 µM did not affect zebrafish mortality, hatching rate, and heart rate compared with the control group. For embryos, PSE caused an increase at 16–32 hpf in zebrafish coiling frequency which could be rescued by serotonin antagonist WAY100635. Similarly, PSE caused an increase in the swimming distance of zebrafish larvae at 120 hpf. PSE also elevated the expression of serotonin (5-HT)-related genes 5-htr1ab and tph2 and dopamine-related gene dbh. Behavioral changes in zebrafish embryos and larvae caused by PSE may be closely associated with increased expression of 5-HT and dopamine-related genes. This may be reflected that the behavioral changes in zebrafish are a possible PSE monitoring indicator.",

keywords = "Behavioral changes, Dopamine, Pseudoephedrine hydrochloride, Serotonin, Zebrafish embryos",

author = "Yini Zhou and Tonglaga Li and Shangzi Zhou and Han Xu and Xiaoyu Yin and Hao Chen and Xuan Ni and Meirong Bai and Wuliji Ao and Jingfeng Yang and Ahmed, {R. G.} and Xuefu Zhang and Shuyin Bao and Jianhua Yu and Kwok, {Kevin W.H.} and Wu Dong",

note = "Funding Information: This work was funded by the Supported by Program for Innovative Research Team in Universities of Inner Mongolia Autonomous Region of China (NMGIRT2216), National Natural Science Foundation of China (8216141082 and 82060672), Natural Science Foundation of Inner Mongolia Autonomous Region of China (2020MS08103 and 2020MS08101); Open Project Program of Inner Mongolia Research Institute of Traditional Mongolian Medicine Engineering Technology, China (MDK2020002), Open project from Key Laboratory of Traditional Mongolian Medicine Research & Development Engineering, State Ethnic Affairs Commission and Ministry of Education (MDK2019069); Open Project Program of Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, China (MDK2018025, MDK2019072, MDK2019074, and MDK 2019077); Startup Project of Doctor scientific research in Inner Mongolia Minzu University of China (BS555 and BS562). Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2022",

month = oct,

doi = "10.1007/s11011-022-01042-0",

language = "English",

volume = "37",

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T1 - Pseudoephedrine hydrochloride causes hyperactivity in zebrafish via modulation of the serotonin pathway

AU - Zhou, Yini

AU - Li, Tonglaga

AU - Zhou, Shangzi

AU - Xu, Han

AU - Yin, Xiaoyu

AU - Chen, Hao

AU - Ni, Xuan

AU - Bai, Meirong

AU - Ao, Wuliji

AU - Yang, Jingfeng

AU - Ahmed, R. G.

AU - Zhang, Xuefu

AU - Bao, Shuyin

AU - Yu, Jianhua

AU - Kwok, Kevin W.H.

AU - Dong, Wu

N1 - Funding Information: This work was funded by the Supported by Program for Innovative Research Team in Universities of Inner Mongolia Autonomous Region of China (NMGIRT2216), National Natural Science Foundation of China (8216141082 and 82060672), Natural Science Foundation of Inner Mongolia Autonomous Region of China (2020MS08103 and 2020MS08101); Open Project Program of Inner Mongolia Research Institute of Traditional Mongolian Medicine Engineering Technology, China (MDK2020002), Open project from Key Laboratory of Traditional Mongolian Medicine Research & Development Engineering, State Ethnic Affairs Commission and Ministry of Education (MDK2019069); Open Project Program of Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, China (MDK2018025, MDK2019072, MDK2019074, and MDK 2019077); Startup Project of Doctor scientific research in Inner Mongolia Minzu University of China (BS555 and BS562). Publisher Copyright: © 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022/10

Y1 - 2022/10

N2 - This study aimed to explore behavioral changes of embryonic and larval zebrafish caused by pseudoephedrine hydrochloride (PSE) and its underlying mechanism. Zebrafish embryos were exposed to 0.5 µM, 2 µM, and 8 µM PSE at 4 h post-fertilization (4 hpf) or 22–23 hpf. Mortality, hatching rate, coiling frequency, heart rate, behavior changes, and related gene expression were observed at different developmental stages. PSE below 8 µM did not affect zebrafish mortality, hatching rate, and heart rate compared with the control group. For embryos, PSE caused an increase at 16–32 hpf in zebrafish coiling frequency which could be rescued by serotonin antagonist WAY100635. Similarly, PSE caused an increase in the swimming distance of zebrafish larvae at 120 hpf. PSE also elevated the expression of serotonin (5-HT)-related genes 5-htr1ab and tph2 and dopamine-related gene dbh. Behavioral changes in zebrafish embryos and larvae caused by PSE may be closely associated with increased expression of 5-HT and dopamine-related genes. This may be reflected that the behavioral changes in zebrafish are a possible PSE monitoring indicator.

AB - This study aimed to explore behavioral changes of embryonic and larval zebrafish caused by pseudoephedrine hydrochloride (PSE) and its underlying mechanism. Zebrafish embryos were exposed to 0.5 µM, 2 µM, and 8 µM PSE at 4 h post-fertilization (4 hpf) or 22–23 hpf. Mortality, hatching rate, coiling frequency, heart rate, behavior changes, and related gene expression were observed at different developmental stages. PSE below 8 µM did not affect zebrafish mortality, hatching rate, and heart rate compared with the control group. For embryos, PSE caused an increase at 16–32 hpf in zebrafish coiling frequency which could be rescued by serotonin antagonist WAY100635. Similarly, PSE caused an increase in the swimming distance of zebrafish larvae at 120 hpf. PSE also elevated the expression of serotonin (5-HT)-related genes 5-htr1ab and tph2 and dopamine-related gene dbh. Behavioral changes in zebrafish embryos and larvae caused by PSE may be closely associated with increased expression of 5-HT and dopamine-related genes. This may be reflected that the behavioral changes in zebrafish are a possible PSE monitoring indicator.

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KW - Dopamine

KW - Pseudoephedrine hydrochloride

KW - Serotonin

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DO - 10.1007/s11011-022-01042-0

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C2 - 35907131

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SN - 0885-7490

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EP - 2568

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JF - Metabolic Brain Disease

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ER -

Pseudoephedrine hydrochloride causes hyperactivity in zebrafish via modulation of the serotonin pathway

Abstract

Keywords

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