Protocol for the sonogenetic stimulation of mouse brain by non-invasive ultrasound

Quanxiang Xian; Zhihai Qiu; Shashwati Kala; Jinghui Guo; Jiejun Zhu; Kin Fung Wong; Sean Shu Yi Guo; Ting Zhu; Xuandi Hou; Lei Sun

doi:10.1016/j.xpro.2021.100393

Protocol for the sonogenetic stimulation of mouse brain by non-invasive ultrasound

Quanxiang Xian
, Zhihai Qiu
, Shashwati Kala
, Jinghui Guo
, Jiejun Zhu
, Kin Fung Wong
, Sean Shu Yi Guo
, Ting Zhu
, Xuandi Hou
, Lei Sun

Research output: Journal article publication › Journal article › Academic research › peer-review

9 Citations (Scopus)

Abstract

Manipulating specific neural activity by targeted ultrasound intervention is a powerful method to gain causal insight into brain functions and treat brain disorders. The technique of sonogenetics enables controlling of cells that are genetically modulated with ultrasound-sensitive ion channels. Here, we detail the preparations, surgical procedures, ultrasound stimulation process, and simultaneous electromyogram (EMG) measurement necessary for successful sonogenetic stimulation in mice. For complete details on the use and execution of this protocol, please refer to Qiu et al. (2020).

Original language	English
Article number	100393
Journal	STAR Protocols
Volume	2
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2021.100393
Publication status	Published - 18 Jun 2021

Keywords

Genetics
Microscopy
Model Organisms
Neuroscience

ASJC Scopus subject areas

General Biochemistry,Genetics and Molecular Biology
General Neuroscience
General Immunology and Microbiology

More information

10.1016/j.xpro.2021.100393

http://hdl.handle.net/10397/90759

Cite this

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title = "Protocol for the sonogenetic stimulation of mouse brain by non-invasive ultrasound",

abstract = "Manipulating specific neural activity by targeted ultrasound intervention is a powerful method to gain causal insight into brain functions and treat brain disorders. The technique of sonogenetics enables controlling of cells that are genetically modulated with ultrasound-sensitive ion channels. Here, we detail the preparations, surgical procedures, ultrasound stimulation process, and simultaneous electromyogram (EMG) measurement necessary for successful sonogenetic stimulation in mice. For complete details on the use and execution of this protocol, please refer to Qiu et al. (2020).",

keywords = "Genetics, Microscopy, Model Organisms, Neuroscience",

author = "Quanxiang Xian and Zhihai Qiu and Shashwati Kala and Jinghui Guo and Jiejun Zhu and Wong, \{Kin Fung\} and Guo, \{Sean Shu Yi\} and Ting Zhu and Xuandi Hou and Lei Sun",

note = "Funding Information: This work was supported by the Hong Kong Research Grants Council General Research Fund (15104520, 15102417, and 15326416), Hong Kong Innovation Technology Fund Mid-stream Research Program (MRP/018/18X), Key-Area Research and Development Program of Guangdong Province (2018B030331001), and internal funding from the Hong Kong Polytechnic University (1-ZE1K and 1-BBAU). The authors would like to thank the facility and technical support from University Research Facility in Behavioral and Systems Neuroscience (UBSN) of the Hong Kong Polytechnic University. Experimental design and conception by Z.H. J.G. S.K. Q.X. and L.S.; investigation by Q.X. S.K. J.G. Z.Q. J.Z. X.H. T.Z. and K.F.W.; manuscript preparation by Q.X. S.K. S.G. and L.S. with input from all other authors. L.S. supervised the research and acquired funding. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2021 The Author(s)",

year = "2021",

month = jun,

day = "18",

doi = "10.1016/j.xpro.2021.100393",

language = "English",

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TY - JOUR

T1 - Protocol for the sonogenetic stimulation of mouse brain by non-invasive ultrasound

AU - Xian, Quanxiang

AU - Qiu, Zhihai

AU - Kala, Shashwati

AU - Guo, Jinghui

AU - Zhu, Jiejun

AU - Wong, Kin Fung

AU - Guo, Sean Shu Yi

AU - Zhu, Ting

AU - Hou, Xuandi

AU - Sun, Lei

N1 - Funding Information: This work was supported by the Hong Kong Research Grants Council General Research Fund (15104520, 15102417, and 15326416), Hong Kong Innovation Technology Fund Mid-stream Research Program (MRP/018/18X), Key-Area Research and Development Program of Guangdong Province (2018B030331001), and internal funding from the Hong Kong Polytechnic University (1-ZE1K and 1-BBAU). The authors would like to thank the facility and technical support from University Research Facility in Behavioral and Systems Neuroscience (UBSN) of the Hong Kong Polytechnic University. Experimental design and conception by Z.H. J.G. S.K. Q.X. and L.S.; investigation by Q.X. S.K. J.G. Z.Q. J.Z. X.H. T.Z. and K.F.W.; manuscript preparation by Q.X. S.K. S.G. and L.S. with input from all other authors. L.S. supervised the research and acquired funding. The authors declare no competing interests. Publisher Copyright: © 2021 The Author(s)

PY - 2021/6/18

Y1 - 2021/6/18

N2 - Manipulating specific neural activity by targeted ultrasound intervention is a powerful method to gain causal insight into brain functions and treat brain disorders. The technique of sonogenetics enables controlling of cells that are genetically modulated with ultrasound-sensitive ion channels. Here, we detail the preparations, surgical procedures, ultrasound stimulation process, and simultaneous electromyogram (EMG) measurement necessary for successful sonogenetic stimulation in mice. For complete details on the use and execution of this protocol, please refer to Qiu et al. (2020).

AB - Manipulating specific neural activity by targeted ultrasound intervention is a powerful method to gain causal insight into brain functions and treat brain disorders. The technique of sonogenetics enables controlling of cells that are genetically modulated with ultrasound-sensitive ion channels. Here, we detail the preparations, surgical procedures, ultrasound stimulation process, and simultaneous electromyogram (EMG) measurement necessary for successful sonogenetic stimulation in mice. For complete details on the use and execution of this protocol, please refer to Qiu et al. (2020).

KW - Genetics

KW - Microscopy

KW - Model Organisms

KW - Neuroscience

UR - https://www.scopus.com/pages/publications/85102498389

U2 - 10.1016/j.xpro.2021.100393

DO - 10.1016/j.xpro.2021.100393

M3 - Journal article

AN - SCOPUS:85102498389

SN - 2666-1667

VL - 2

JO - STAR Protocols

JF - STAR Protocols

IS - 2

M1 - 100393

ER -

Protocol for the sonogenetic stimulation of mouse brain by non-invasive ultrasound

Abstract

Keywords

ASJC Scopus subject areas

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