Protocol for the sonogenetic stimulation of mouse brain by non-invasive ultrasound

Quanxiang Xian, Zhihai Qiu, Shashwati Kala, Jinghui Guo, Jiejun Zhu, Kin Fung Wong, Sean Shu Yi Guo, Ting Zhu, Xuandi Hou, Lei Sun

Research output: Journal article publicationJournal articleAcademic researchpeer-review

2 Citations (Scopus)


Manipulating specific neural activity by targeted ultrasound intervention is a powerful method to gain causal insight into brain functions and treat brain disorders. The technique of sonogenetics enables controlling of cells that are genetically modulated with ultrasound-sensitive ion channels. Here, we detail the preparations, surgical procedures, ultrasound stimulation process, and simultaneous electromyogram (EMG) measurement necessary for successful sonogenetic stimulation in mice. For complete details on the use and execution of this protocol, please refer to Qiu et al. (2020).

Original languageEnglish
Article number100393
JournalSTAR Protocols
Issue number2
Publication statusPublished - 18 Jun 2021


  • Genetics
  • Microscopy
  • Model Organisms
  • Neuroscience

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)


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