@article{a86c08483abe41a18c190a824821a578,
title = "Protocol for the sonogenetic stimulation of mouse brain by non-invasive ultrasound",
abstract = "Manipulating specific neural activity by targeted ultrasound intervention is a powerful method to gain causal insight into brain functions and treat brain disorders. The technique of sonogenetics enables controlling of cells that are genetically modulated with ultrasound-sensitive ion channels. Here, we detail the preparations, surgical procedures, ultrasound stimulation process, and simultaneous electromyogram (EMG) measurement necessary for successful sonogenetic stimulation in mice. For complete details on the use and execution of this protocol, please refer to Qiu et al. (2020).",
keywords = "Genetics, Microscopy, Model Organisms, Neuroscience",
author = "Quanxiang Xian and Zhihai Qiu and Shashwati Kala and Jinghui Guo and Jiejun Zhu and Wong, {Kin Fung} and Guo, {Sean Shu Yi} and Ting Zhu and Xuandi Hou and Lei Sun",
note = "Funding Information: This work was supported by the Hong Kong Research Grants Council General Research Fund (15104520, 15102417, and 15326416), Hong Kong Innovation Technology Fund Mid-stream Research Program (MRP/018/18X), Key-Area Research and Development Program of Guangdong Province (2018B030331001), and internal funding from the Hong Kong Polytechnic University (1-ZE1K and 1-BBAU). The authors would like to thank the facility and technical support from University Research Facility in Behavioral and Systems Neuroscience (UBSN) of the Hong Kong Polytechnic University. Experimental design and conception by Z.H. J.G. S.K. Q.X. and L.S.; investigation by Q.X. S.K. J.G. Z.Q. J.Z. X.H. T.Z. and K.F.W.; manuscript preparation by Q.X. S.K. S.G. and L.S. with input from all other authors. L.S. supervised the research and acquired funding. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2021 The Author(s)",
year = "2021",
month = jun,
day = "18",
doi = "10.1016/j.xpro.2021.100393",
language = "English",
volume = "2",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",
}