Proteomic study of a model causative agent of harmful algal blooms, Prorocentrum triestinum II: The use of differentially expressed protein profiles under different growth phases and growth conditions for bloom prediction

Leo Lai Chan, Ivor John Hodgkiss, Jennifer Man Fan Wan, John Hon Kei Lum, Abby Sin Chi Mak, Wai Hung Sit, Chun Lap Samuel Lo

Research output: Journal article publicationJournal articleAcademic researchpeer-review

32 Citations (Scopus)

Abstract

Simultaneous comparison of differentially expressed protein profiles of Prorocentrum triestinum grown under different growth phases and growth conditions indicated the presence of phase-specific and stress-responsive proteins, respectively. Correlation studies on these proteins in relation to cell division phasing patterns and to models of phytoplankton growth inferred the possible functions. Most notable among these proteins were groups of proteins thought to trigger or mediate cells through specific phases of division of this alga, e.g., BP1, BP2, PB1, PB2, and PB3. Other proteins (e.g., group 1 proteins) thought to be responsible for maintaining and supporting cell concentration under adverse conditions were found. Furthermore, another group of proteins (group 2 proteins) thought to be stress-responsive were also detected. Taken overall, these differentially expressed proteins provided important information for uncovering various protective and adaptive mechanisms in the dinoflagellate's life cycle. These proteins have the potential to serve as "indicator proteins" for rapid assessment of the nutritional or metabolic status of these phytoplankton cells, and monitoring the differential expression of these phase-specific proteins and stress-specific proteins could be an important biomarker for bloom prediction.
Original languageEnglish
Pages (from-to)3214-3226
Number of pages13
JournalProteomics
Volume4
Issue number10
DOIs
Publication statusPublished - 1 Oct 2004

Keywords

  • Cell cycle
  • Environmental stress
  • Growth phases
  • Harmful algal bloom (HAB) species
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this