@inbook{61fece3df74f4704a5f689678c9dd9ff,
title = "Proteomic Analysis of Secreted Vesicle Proteins from the Plant Cells",
abstract = "A working pipeline for proteomic analysis of secreted vesicle proteins from the plant cells has been developed using urea and mass spectrometry-compatible detergent RapiGest SF, where vesicles could be efficiently lysed and membrane-bound proteins could be efficiently dissolved and digested. The vesicle lysis and the protein digestion procedures are performed within one tube to minimize the protein loss. The protein digest is analyzed using LC-MS/MS after desalting with an SPE spin column.",
keywords = "LC-MS/MS, Plant cells, Proteomic analysis, Vesicle proteins",
author = "Haidi Yin and Jianying Wang and Yao, \{Zhong Ping\}",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.",
year = "2024",
month = aug,
day = "9",
doi = "10.1007/978-1-0716-4059-3\_5",
language = "English",
volume = "2841",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "67--73",
booktitle = "Methods in Molecular Biology",
}