TY - JOUR
T1 - Protein-Substrate Adhesion in Microcontact Printing Regulates Cell Behavior
AU - Hu, Shuhuan
AU - Chen, Ting Hsuan
AU - Zhao, Yanhua
AU - Wang, Zuankai
AU - Lam, Raymond H.W.
N1 - Funding Information:
We acknowledge financial support from the National Natural Science Foundation of China (project no. 31500758), a General Research Grant (project no. 11206014), the Collaborative Research Fund (project no. C1013-15GF) of the Hong Kong Research Grant Council, the Croucher Foundation (startup grant), and the City University of Hong Kong (project no. 7004602).
Publisher Copyright:
© 2018 American Chemical Society.
PY - 2018/1/30
Y1 - 2018/1/30
N2 - Microcontact printing (μCP) is widely used to create patterns of biomolecules essential for studies of cell mechanics, migration, and tissue engineering. However, different types of μCPs may create micropatterns with varied protein-substrate adhesion, which may change cell behaviors and pose uncertainty in result interpretation. Here, we characterize two μCP methods for coating extracellular matrix (ECM) proteins (stamp-off and covalent bond) and demonstrate for the first time the important role of protein-substrate adhesion in determining cell behavior. We found that, as compared to cells with weaker traction force (e.g., endothelial cells), cells with strong traction force (e.g., vascular smooth muscle cells) may delaminate the ECM patterns, which reduced cell viability as a result. Importantly, such ECM delamination was observed on patterns by stamp-off but not on the patterns by covalent bonds. Further comparisons of the displacement of the ECM patterns between the normal VSMCs and the force-reduced VSMCs suggested that the cell traction force plays an essential role in this ECM delamination. Together, our results indicated that μCPs with insufficient adhesion may lead to ECM delamination and cause cell death, providing new insight for micropatterning in cell-biomaterial interaction on biointerfaces.
AB - Microcontact printing (μCP) is widely used to create patterns of biomolecules essential for studies of cell mechanics, migration, and tissue engineering. However, different types of μCPs may create micropatterns with varied protein-substrate adhesion, which may change cell behaviors and pose uncertainty in result interpretation. Here, we characterize two μCP methods for coating extracellular matrix (ECM) proteins (stamp-off and covalent bond) and demonstrate for the first time the important role of protein-substrate adhesion in determining cell behavior. We found that, as compared to cells with weaker traction force (e.g., endothelial cells), cells with strong traction force (e.g., vascular smooth muscle cells) may delaminate the ECM patterns, which reduced cell viability as a result. Importantly, such ECM delamination was observed on patterns by stamp-off but not on the patterns by covalent bonds. Further comparisons of the displacement of the ECM patterns between the normal VSMCs and the force-reduced VSMCs suggested that the cell traction force plays an essential role in this ECM delamination. Together, our results indicated that μCPs with insufficient adhesion may lead to ECM delamination and cause cell death, providing new insight for micropatterning in cell-biomaterial interaction on biointerfaces.
UR - http://www.scopus.com/inward/record.url?scp=85041445378&partnerID=8YFLogxK
U2 - 10.1021/acs.langmuir.7b02935
DO - 10.1021/acs.langmuir.7b02935
M3 - Journal article
C2 - 29304548
AN - SCOPUS:85041445378
SN - 0743-7463
VL - 34
SP - 1750
EP - 1759
JO - Langmuir
JF - Langmuir
IS - 4
ER -