Protein identification by automated nanospray mass spectrometry-"zoomscan walking"

Matt J. Sweeney, Chi Bun Ko, Stephen S M Chung, Christoph W. Turck

Research output: Journal article publicationJournal articleAcademic researchpeer-review

5 Citations (Scopus)

Abstract

Nanospray mass spectrometry is an important tool for highsensitivity protein identification.A disadvantage of nanospray infusion is that the operator must search for peptide ions from a protein digest for subsequent collision-induced fragmentation. In order to make the nanospray method less operator-dependent, we have implemented an automated procedure using commonly available tools. Protein digests are subjected to unattended nanospray analysis using an automatic "zoomscan walking" procedure. The identification of eight proteins from an SDS gel using automated "zoomscan walking" is shown as an example of the utility of the method.
Original languageEnglish
Pages (from-to)43-48
Number of pages6
JournalJournal of Biomolecular Techniques
Volume13
Issue number2
Publication statusPublished - 1 Jun 2002
Externally publishedYes

Keywords

  • Mass spectrometry
  • Nanospray
  • Zoomscan

ASJC Scopus subject areas

  • Molecular Biology

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