Abstract
The effects of bis(7)-tacrine, a novel acetylcholinesterase inhibitor, on ischemia-induced cell death and apoptosis were investigated in primary cerebral cortical astrocytes of mice. Following a 6 h in vitro ischemic incubation of the cultures, a marked decrease in the percentage of viable cells was observed by lactate dehydrogenase (LDH) release assay. Furthermore, using bisbenzimide staining, we determined that approximately 65% of the cells underwent apoptosis. Treatment with bis(7)-tacrine (1-10 nM) during ischemic incubation effectively inhibited the ischemia-induced apoptosis, as demonstrated by morphological and biochemical tests. Our results demonstrated that bis(7)-tacrine could protect astrocytes against ischemia-induced cell injury, indicating that the drug might be beneficial for the treatment of vascular dementia, in addition to Alzheimer's disease.
Original language | English |
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Pages (from-to) | 95-98 |
Number of pages | 4 |
Journal | Neuroscience Letters |
Volume | 288 |
Issue number | 2 |
DOIs | |
Publication status | Published - 14 Jul 2000 |
Externally published | Yes |
Keywords
- Acetylcholinesterase inhibitor
- Astrocyte
- Bis(7)-tacrine
- Ischemia
ASJC Scopus subject areas
- General Neuroscience