Protection against ischemic injury in primary cultured astrocytes of mouse cerebral cortex by bis(7)-tacrine, a novel anti-Alzheimer's agent

Yifan Han, Dong Cheng Wu, Xiao Qiu Xiao, Priscilla M.Y. Chen, Wilson Chung, Nelson T.K. Lee, Yuan Ping Pang, Paul R. Carlier

Research output: Journal article publicationJournal articleAcademic researchpeer-review

37 Citations (Scopus)

Abstract

The effects of bis(7)-tacrine, a novel acetylcholinesterase inhibitor, on ischemia-induced cell death and apoptosis were investigated in primary cerebral cortical astrocytes of mice. Following a 6 h in vitro ischemic incubation of the cultures, a marked decrease in the percentage of viable cells was observed by lactate dehydrogenase (LDH) release assay. Furthermore, using bisbenzimide staining, we determined that approximately 65% of the cells underwent apoptosis. Treatment with bis(7)-tacrine (1-10 nM) during ischemic incubation effectively inhibited the ischemia-induced apoptosis, as demonstrated by morphological and biochemical tests. Our results demonstrated that bis(7)-tacrine could protect astrocytes against ischemia-induced cell injury, indicating that the drug might be beneficial for the treatment of vascular dementia, in addition to Alzheimer's disease.
Original languageEnglish
Pages (from-to)95-98
Number of pages4
JournalNeuroscience Letters
Volume288
Issue number2
DOIs
Publication statusPublished - 14 Jul 2000
Externally publishedYes

Keywords

  • Acetylcholinesterase inhibitor
  • Astrocyte
  • Bis(7)-tacrine
  • Ischemia

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this