Protection against 1-methyl-4-phenylpyridinium ion (MPP +)- induced apoptosis by water extract of ginseng (Panax ginseng C.A. Meyer) in SH-SY5Y cells

Shengquan Hu, Renwen Han, Shinghung Mak, Yifan Han

Research output: Journal article publicationJournal articleAcademic researchpeer-review

53 Citations (Scopus)

Abstract

Ethnopharmacological relevance: The present study investigates the protective effects of water extract of ginseng (Panax ginseng C.A. Meyer) against 1-methyl-4-phenylpyridinium ion (MPP+)-induced cytotoxicity in SH-SY5Y human neuroblastoma cells and explores the underlying mechanisms. The approach may be used for screening therapeutic agents for degenerative disorders such as Parkinson's disease. Materials and methods: SH-SY5Y human neuroblastoma cells were used to analyze the protective effects of water extract of ginseng (WEG) against multiple parameters such as MPP+-induced viability, oxidative injury, expression of Bax, Bcl-2, cytochrome c and cleaved caspase-3. Results: WEG exerted inhibitory effect on cell death, overproduction of ROS, elevated Bax/Bcl-2 ratio, release of cytochrome c and activation of caspase-3 expression in MPP+-treated SH-SY5Y cells. Conclusions: WEG exhibited significant protective effects against MPP+-induced cytotoxicity in SH-SY5Y cells possibly through the suppression of ROS generation and the inhibition of mitochondria-dependent apoptotic pathway.
Original languageEnglish
Pages (from-to)34-42
Number of pages9
JournalJournal of Ethnopharmacology
Volume135
Issue number1
DOIs
Publication statusPublished - 26 Apr 2011

Keywords

  • Ginseng (Panax ginseng C.A. Meyer)
  • Mitochondrial dysfunction
  • MPP +
  • Parkinson's disease (PD)
  • Reactive oxygen species (ROS)
  • Water extract of ginseng (WEG)

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

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