Promotion of Salvia miltiorrhiza hairy root growth and tanshinone production by polysaccharide-protein fractions of plant growth-promoting rhizobacterium Bacillus cereus

Jiang Lin Zhao, Li Gang Zhou, Jianyong Wu

Research output: Journal article publicationJournal articleAcademic researchpeer-review

59 Citations (Scopus)

Abstract

This study was to examine the effects of polysaccharides from a plant growth-promoting rhizobacterium (PGPR) Bacillus cereus on the growth and tanshinone production of Salvia miltiorrhiza hairy roots. A polysaccharide fraction designated BPS was isolated from the hot water extract of B. cereus cells by ethanol precipitation. BPS applied to the root culture at 100-400mgl-1a few days before the stationary growth phase stimulated the tanshinone accumulation of roots by about 7-fold (1.59mgg-1versus 0.19mgg-1) and also notably promoted the root growth (15% increase in biomass). BPS was a polysaccharide-protein complex containing about 27% protein, which is essential for root growth promotion. BPS was separated by ultrafiltration into two molecular weight (MW) fractions, of which the high MW fraction (∼35.8kDa) with higher protein content (∼31%) promoted the root growth while the lower MW fraction with lower protein content (∼17%) suppressed the growth. The results suggest that the polysaccharide portion of BPS was responsible for stimulating the tanshinone accumulation while the protein portion was responsible for promoting the hairy root growth. Polysaccharides from PGPR are potential sources of active elicitors and growth-promoting agents for plant roots in culture.
Original languageEnglish
Pages (from-to)1517-1522
Number of pages6
JournalProcess Biochemistry
Volume45
Issue number9
DOIs
Publication statusPublished - 1 Sep 2010

Keywords

  • Bacillus cereus
  • Elicitors
  • Hairy root culture
  • Polysaccharides
  • Salvia miltiorrhiza
  • Tanshinones

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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