Prognostic significance of phosphorylated RON in esophageal squamous cell carcinoma

Marco K C Hui, Kenneth K Y Lai, Kwok Wah Chan, John M. Luk, Nikki P. Lee, Yvonne Chung, Leo C. Cheung, Gopesh Srivastava, Sai Wah Tsao, Cheuk On Tang, Simon Law

Research output: Journal article publicationJournal articleAcademic researchpeer-review

7 Citations (Scopus)

Abstract

Esophageal squamous cell carcinoma (ESCC) is the most common type of esophageal cancer. RON is a transmembrane receptor overexpressed in various cancers; however, the clinical significance of its phosphorylated form (pRON) is not fully deciphered. This report is the first to investigate the expression and clinical significance of pRON in human ESCC. Quantitative polymerase chain reaction revealed an up-regulation of RON mRNA in 70% (7/10) of ESCC tissues when compared to the adjacent nontumor tissues. An overexpression of pRON protein was found in most of the ESCC cell lines studied (4/5) when compared to two non-neoplastic esophageal epithelial cells using immunoblot. In 64 ESCC tissues, pRON was localized at the cell membrane, cytoplasm and nucleus in 15 (23.4%), 63 (98.4%) and 61 (95.3%) cases using immunohistochemistry. Patients having high expression of cytoplasmic pRON significantly associated with shorter median survival when compared to those with low expression (25.41 months vs. 14.43 months), suggesting cytoplasmic pRON as a potential marker for poor prognosis in ESCC patients.
Original languageEnglish
Pages (from-to)1699-1706
Number of pages8
JournalMedical Oncology
Volume29
Issue number3
DOIs
Publication statusPublished - 1 Sep 2012

Keywords

  • Esophageal squamous cell carcinoma
  • Median survival after surgery
  • Overexpression
  • Phosphorylated RON
  • Poor prognostic marker

ASJC Scopus subject areas

  • Oncology
  • Cancer Research
  • Hematology

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