Progenitor cells derived from drain waste product of open-heart surgery in children

Tak Wah Wong, Chung Dann Kan, Wen Tai Chiu, Kin Lam Fok, Ye Chun Ruan, Xiaohua Jiang, Junjiang Chen, Chiu Ching Kao, I. Yu Chen, Hui Chun Lin, Chia Hsuan Chou, Chou Wen Lin, Chun Keung Yu, Stephanie Tsao, Yi Ping Lee, Hsiao Chang Chan, Jieh Neng Wang

Research output: Journal article publicationJournal articleAcademic researchpeer-review

1 Citation (Scopus)

Abstract

Human cardiac progenitor cells isolated from the same host may have advantages over other sources of stem cells. The aim of this study is to establish a new source of human progenitor cells collected from a waste product, pericardiac effusion fluid, after open-heart surgery in children with congenital heart diseases. The fluid was collected every 24 h for 2 days after surgery in 37 children. Mononuclear cells were isolated and expanded in vitro. These pericardial effusion-derived progenitor cells (PEPCs) exhibiting cardiogenic lineage markers, were highly proliferative and enhanced angiogenesis in vitro. Three weeks after stem cell transplantation into the ischemic heart in mice, cardiac ejection fraction was improved significantly without detectable progenitor cells. Gene expression profiles of the repaired hearts revealed activation of several known repair mechanisms including paracrine effects, cell migration, and angiogenesis. These progenitor cells may have the potential for heart regeneration.

Original languageEnglish
Article number1028
JournalJournal of Clinical Medicine
Volume8
Issue number7
DOIs
Publication statusPublished - Jul 2019

Keywords

  • Cardiac surgery
  • Congenital heart disease
  • Open heart
  • Progenitor cells
  • Regeneration
  • Stem cells

ASJC Scopus subject areas

  • Medicine(all)

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