Probing the mechanobiological properties of human embryonic stem cells in cardiac differentiation by optical tweezers

Youhua Tan, Chi wing Kong, Shuxun Chen, Shuk Han Cheng, Ronald A. Li, Dong Sun

Research output: Journal article publicationJournal articleAcademic researchpeer-review

52 Citations (Scopus)

Abstract

Human embryonic stem cells (hESC) and hESC-derived cardiomyocytes (hESC-CM) hold great promise for the treatment of cardiovascular diseases. However the mechanobiological properties of hESC and hESC-CM remains elusive. In this paper, we examined the dynamic and static micromechanical properties of hESC and hESC-CM, by manipulating via optical tweezers at the single-cell level. Theoretical approaches were developed to model the dynamic and static mechanical responses of cells during optical stretching. Our experiments showed that the mechanical stiffness of differentiated hESC-CM increased after cardiac differentiation. Such stiffening could associate with increasingly organized myofibrillar assembly that underlines the functional characteristics of hESC-CM. In summary, our findings lay the ground work for using hESC-CMs as models to study mechanical and contractile defects in heart diseases.
Original languageEnglish
Pages (from-to)123-128
Number of pages6
JournalJournal of Biomechanics
Volume45
Issue number1
DOIs
Publication statusPublished - 3 Jan 2012
Externally publishedYes

Keywords

  • Biomechanics
  • Cardiac differentiation
  • Cell manipulation
  • Human embryonic stem cell
  • Optical tweezers

ASJC Scopus subject areas

  • Biophysics
  • Orthopedics and Sports Medicine
  • Biomedical Engineering
  • Rehabilitation

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